Human LCoR Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB7534

R&D Systems, part of Bio-Techne
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MAB7534
MAB7534-SP
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 731229

View all LCoR Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human LCoR
Asn110-Gln338
Accession # Q96JN0

Specificity

Detects human LCoR in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human LCoR Antibody

LCoR antibody in MCF-7 Human Cell Line by Immunocytochemistry (ICC).

LCoR in MCF‑7 Human Cell Line.

LCoR was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human LCoR Monoclonal Antibody (Catalog # MAB7534) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human LCoR Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed MCF‑7 human breast cancer cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LCoR

LCoR (ligand-dependent corepressor), also known as MLR2 (Mblk1-related protein 2), is a 47-50 kDa nuclear phosphoprotein that is a corepressor of ligand-dependent transcriptional activation by nuclear receptors such as estrogen and progesterone receptors. It is widely expressed throughout fetal development and in the adult. The 433 amino acid (aa) LCoR contains two N-terminal PxDLS nuclear receptor interaction motifs, a central HDAC interaction motif, a nuclear localization signal, and a C‑terminal Helix-Turn-Helix motif. Alternate splicing produces a truncated 406 aa variant. Within the region used as an immunogen, human LCoR shares 97% aa identity with mouse and rat LCoR.

Long Name

Ligand-dependent Nuclear Receptor Corepressor

Alternate Names

MLR2

Entrez Gene IDs

84458 (Human); 212391 (Mouse); 365462 (Rat)

Gene Symbol

LCOR

UniProt

Q96JN0 (Human)

Additional LCoR Products

Product Documents for Human LCoR Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human LCoR Antibody

For research use only

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