Human LGI2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7334
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 748914
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human LGI2
Gln29-Ser276
Accession # Q8N0V4
Gln29-Ser276
Accession # Q8N0V4
Specificity
Detects human LGI2 in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human LGI2 Antibody
Detection of Human LGI2 by Western Blot.
Western blot shows lysates of HEK293 human embryonic kidney cell line, SH-SY5Y human neuroblastoma cell line, and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human LGI2 Monoclonal Antibody (Catalog # MAB7334) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for LGI2 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
LGI2 in HepG2 Human Cell Line.
LGI2 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human LGI2 Monoclonal Antibody (Catalog # MAB7334) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counter-stained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human LGI2 Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample:
Sample:
Immersion fixed HepG2 human hepatocellular carcinoma cell line
Western Blot
2 µg/mL
Sample: HEK293 human embryonic kidney cell line, SH‑SY5Y human neuroblastoma cell line, and HepG2 human hepatocellular carcinoma cell line
Sample: HEK293 human embryonic kidney cell line, SH‑SY5Y human neuroblastoma cell line, and HepG2 human hepatocellular carcinoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LGI2
Long Name
Leucine-rich, Glioma-inactivated 2
Alternate Names
LGIL2
Gene Symbol
LGI2
UniProt
Additional LGI2 Products
Product Documents for Human LGI2 Antibody
Product Specific Notices for Human LGI2 Antibody
For research use only
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