Skip to main content

Human LIGHT/TNFSF14 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB6642

Recombinant Monoclonal Antibody.
R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
Loading...
MAB6642-100
MAB6642-SP

Key Product Details

Species Reactivity

Human

Applications

ELISA

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 Clone # 115515R

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human LIGHT/TNFSF14
Asp74-Val240
Accession # O46557

Specificity

Detects human LIGHT/TNFSF14 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human LIGHT/TNFSF14 Antibody

Human LIGHT/TNFSF14 Antibody in ELISA Standard Curve.

Human LIGHT/TNFSF14 ELISA Standard Curve.

Recombinant Human LIGHT/TNFSF14 protein was serially diluted 2-fold and captured by Mouse Anti-Human LIGHT/TNFSF14 Monoclonal Antibody (Catalog # MAB6642) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human LIGHT/TNFSF14 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF664) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Applications for Human LIGHT/TNFSF14 Antibody

Application
Recommended Usage

ELISA

This antibody functions as an ELISA capture antibody when paired with Goat Anti-Human LIGHT/TNFSF14 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF664).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human LIGHT/TNFSF14 DuoSet ELISA Kit (Catalog # DY664) for convenient development of a sandwich ELISA or the Human LIGHT/TNFSF14 Quantikine ELISA Kit (Catalog # DLIT00) for a complete optimized ELISA.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty
Loading...

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LIGHT/TNFSF14

Human LIGHT, also known as TNFSF14, is a type II membrane protein that is a member of the TNF superfamily. LIGHT is an acronym which stands for "is homologous to lymphotoxins, exhibits inducible expression, and competes with HSV glycoprotein D for HVEM, a receptor expressed by T lymphocytes". LIGHT has also been called HVEM-L and LT-gamma. LIGHT is a 240 amino acid (aa) protein that contains a 37 aa cytoplasmic domain, a 22 aa transmembrane region, and a 181 aa extracellular domain. Similar to other TNF ligand family members, LIGHT is predicted to assemble as a homotrimer. LIGHT is produced by activated T cells and was first identified by its ability to compete with HSV glycoprotein D for HVEM binding. LIGHT has also been shown to bind to the lymphotoxin beta receptor (LT betaR) and the decoy receptor (DcR3/TR6). LIGHT overexpression in tumor cells induces apoptosis, which can be enhanced by IFN-gamma.

References

  1. Mauri, D.N. et al. (1998) Immunity 8:21.
  2. Zhai, Y. et al. (1998) J. Clin. Invest. 102:1142.
  3. Harrop, J.A. et al. (1998) J. Biol. Chem. 273:27548.
  4. Yu, K-Y. et al. (1999) J. Biol. Chem. 274:13733.

Long Name

TNF Ligand Superfamily Member 14

Alternate Names

CD258, TNFSF14

Entrez Gene IDs

8740 (Human); 50930 (Mouse)

Gene Symbol

TNFSF14

UniProt

Additional LIGHT/TNFSF14 Products

Product Documents for Human LIGHT/TNFSF14 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human LIGHT/TNFSF14 Antibody

For research use only

Loading...
Loading...
Loading...
Loading...