Detects human LILRA2/CD85h/ILT1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) ILT2, 3, 4, 5, rhLIR6, or rhLIR8 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Human LILRA2/CD85h/ILT1 Antibody

Detection of LILRA2/CD85h/ILT1 in Human PBMC by Flow Cytometry.

Human peripheral blood monocytes were stained with Human LILRA2/CD85h/ILT1 Monoclonal Antibody (Catalog # MAB6364, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0101B).

Applications for Human LILRA2/CD85h/ILT1 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/10⁶ cells
Sample: Human peripheral blood monocytes

Reviewed Applications

Read 1 review rated 4 using MAB6364 in the following applications:

Immunofluorescence-Frozen (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LILRA2/CD85h/ILT1

Immune-like transcript 1 (ILT1), also known as leukocyte immunoglobulin-like receptor 7 (LIR-7), leukocyte immunoglobulin-like receptor subfamily A member 2 (LILRA2), and CD85H, is a 51 kDa (unglycosylated) type I transmembrane glycoprotein and member of the leukocyte immunoglobulin-like receptor family. Human ILT1 is synthesized as a 483 amino acid (aa) precursor that contains a 23 aa signal sequence, a 426 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 13 aa cytoplasmic region. The ECD contains four Ig-like C2-type domains and seven potential sites for N-linked glycosylation. A splice variant produces a second isoform that has a one aa substitution for aa 419-436 in the longer form. There are no murine orthologs for human ILT1. ILT1 may act as a receptor for class I MHC antigens.

Long Name

Leukocyte Immunoglobulin-like Receptor, Subfamily A (with TM domain), Member 2

Alternate Names

CD85h, ILT1, LIR7

Entrez Gene IDs

11027 (Human)

Gene Symbol

LILRA2

UniProt

Q8N149 (Human)

Additional LILRA2/CD85h/ILT1 Products

Product Documents for Human LILRA2/CD85h/ILT1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human LILRA2/CD85h/ILT1 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human LILRA2/CD85h/ILT1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB6364

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

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Product Specifications

Immunogen

Specificity