Human LILRA4/CD85g/ILT7 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB62871

R&D Systems, part of Bio-Techne
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MAB62871-100
MAB62871-SP
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Key Product Details

Species Reactivity

Human

Applications

Neutralization

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 656656

View all LILRA4/CD85g/ILT7 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line, NS0­derived human LILRA4/CD85g/ILT7
Glu24-Asn446
Accession # P59901

Specificity

Detects human LILRA4/CD85g/ILT7 in ELISA. No cross-reactivity with human LILRA1, LILRA2, LILRA3, LILRA5, LILRA6, LILRB2 and LILRB3 with ELISA was observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Applications for Human LILRA4/CD85g/ILT7 Antibody

Application
Recommended Usage

Neutralization

In a functional ELISA binding assay, 0.1-1.2 ug/mL of this antibody will block 50% of the binding of  0.3 ug/mL of Recombinant Human Angiopoietin‑like Protein 7/ANGPTL7 (Catalog # 914-AN) when Recombinant Human LILRA4/CD85g/ILT7 (Catalog # 8914-T4) is immobilized at 1 ug/mL (100 µL/well). At 10 ug/mL, this antibody will block >90% of the binding.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LILRA4/CD85g/ILT7

LILRA4, also known as ILT7 and CD85g, is an approximately 60-70 kDa variably glycosylated transmembrane protein that regulates immune cell activation (1). Mature human LILRA4 consists of a 423 amino acid (aa) extracellular domain (ECD) with four immunoglobulin-like domains, a 21 aa transmembrane segment, and a 32 aa cytoplasmic domain (2). Alternative splicing generates an additional isoform that lacks the signal peptide and a portion of the first Ig-like domain. LILRA4 is expressed on plasmacytoid dendritic cells (pDC) but is down-regulated in response to TLR9 signaling (3-5). Antibody mediated crosslinking of LILRA4 on pDC inhibits the production of type I interferons following TLR7 or TLR9 stimulation (3, 4, 6). It also blocks the up-regulation of CCR7 but enhances the up-regulation of Integrin  beta7 on TLR7/9-stimulated pDC (6). LILRA4 associates with the ITAM-containing adaptor protein Fc epsilon RI gamma (3, 4, 6), and this complex binds to cell surface BST2/Tetherin which is expressed on monocytes, plasmacytoid and myeloid dendritic cells, B cells, and activated CD4+ and CD8+ T cells (5, 8). This interaction inhibits the TLR-induced pDC production of type I interferons, IL-6, and TNF-alpha (8).

References

  1. Thomas, R. et al. (2010) Clin. Rev. Allergy Immunol. 38:159.
  2. Young, N.T. et al. (2001) Immunogenetics 53:270.
  3. Cho, M. et al. (2008) Int. Immunol. 20:155.
  4. Cao, W. et al. (2006) J. Exp. Med. 203:1399.
  5. Tavano, B. et al. (2013) J. Immunol. 190:2622.
  6. Tsukamoto, N. et al. (2009) Clin. Cancer Res. 15:5733.
  7. Tavano, B. and A. Boasso (2014) PLoS ONE 9:e89414.
  8. Cao, W. et al. (2009) J. Exp. Med. 206:1603.

Long Name

Leukocyte Immunoglobulin-like Receptor, Subfamily A (with TM domain), Member 4

Alternate Names

CD85g, ILT7

Entrez Gene IDs

23547 (Human)

Gene Symbol

LILRA4

UniProt

P59901 (Human)

Additional LILRA4/CD85g/ILT7 Products

Product Documents for Human LILRA4/CD85g/ILT7 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human LILRA4/CD85g/ILT7 Antibody

For research use only

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