Detects human LILRA4/CD85g/ILT7 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) LILRA5, rhILT2, 3, 4, 5, or 6 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2A

Scientific Data Images for Human LILRA4/CD85g/ILT7 Antibody

Detection of LILRA4/CD85g/ILT7 in Human PBMCs by Flow Cytometry.

Human peripheral blood monocytes were labeled with Mouse Anti-Human LILRA4/CD85g/ILT7 Monoclonal Antibody (Catalog # MAB6287) followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0101B) and Human DLEC/CLEC4C/BDCA-2 Fluorescein-conjugated Polyclonal Antibody (Catalog # FAB1376F). Quadrant markers were set based on control antibody staining (Catalog # MAB003).

Applications for Human LILRA4/CD85g/ILT7 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/10⁶ cells
Sample: Human peripheral blood monocytes

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LILRA4/CD85g/ILT7

LILRA4, also known as ILT7 and CD85g, is a 499 amino acid (aa) type I transmembrane glycoprotein that contains four Ig-like domains in its extracellular region. LILRA4 is selectively expressed on plasmacytoid dendritic cells (pDC). It binds to BST2/CD317 on bone marrow stromal cells and associates with the gamma subunit of Fc epsilon RI. This receptor complex transmits signals that inhibit the TLR-induced production of type I interferon. The LILRA4-mediated interactions between pDC and tumor cells similarly inhibit the production of proinflammatory cytokines and contribute to reduced anti-tumor immune responses. Alternate splicing of human LILRA4 generates an isoform that lacks the N-terminal 43 aa of the extracellular domain.

Long Name

Leukocyte Immunoglobulin-like Receptor, Subfamily A (with TM domain), Member 4

Alternate Names

CD85g, ILT7

Entrez Gene IDs

23547 (Human)

Gene Symbol

LILRA4

UniProt

P59901 (Human)

Additional LILRA4/CD85g/ILT7 Products

Product Documents for Human LILRA4/CD85g/ILT7 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human LILRA4/CD85g/ILT7 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human LILRA4/CD85g/ILT7 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB6287

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

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Product Specifications

Immunogen

Specificity