HEK293 human embryonic kidney cell line transfected with human LILRA6/CD85b and eGFP were stained with either (A) Mouse Anti-Human LILRA6/CD85b Monoclonal Antibody (Catalog # MAB8656) or (B) Mouse IgG₁Isotype Control (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Detection of LILRA6/CD85b in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human LILRA6/CD85b Monoclonal Antibody (Catalog # MAB8656, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Applications for Human LILRA6/CD85b Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 human embryonic kidney cell line transfected with human LILRA6/CD85b and eGFP, and Human peripheral blood mononuclear cells (PBMCs)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LILRA6/CD85b

LILRA6 (Leukocyte immunoglobulin-like receptor subfamily A, member 6) is also known as ILT5 and CD85b. The leukocyte immunoglobulin-like receptors (LILR) are a family of receptors possessing extracellular immunoglobulin domains. They are also known as ILTs and can exert immunomodulatory effects on a wide range of immune cells. LILRA6 is a highly polymorphic surface molecule that is one of the targets of dendritic cell-reactive antibodies.

Long Name

Leukocyte Immunoglobulin-like Receptor A6

Alternate Names

7M1, CD85b, ILT8, Pira3

Entrez Gene IDs

79168 (Human); 18726 (Mouse)

Gene Symbol

LILRA6

UniProt

Q6PI73

Additional LILRA6/CD85b Products

Product Documents for Human LILRA6/CD85b Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human LILRA6/CD85b Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human LILRA6/CD85b Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB8656

Key Product Details

Species Reactivity

Validated:

Applications

Label

Antibody Source

View all LILRA6/CD85b Antibodies »

Product Summary for Human LILRA6/CD85b Antibody

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human LILRA6/CD85b Antibody

Detection of LILRA6/CD85b in HEK293 Human Cell Line Transfected with Human LILRA6/CD85b and eGFP by Flow Cytometry.