Human Lipin 1 Biotinylated Antibody

Name: Human Lipin 1 Biotinylated Antibody
Brand: R&D Systems
SKU: BAF3885

R&D Systems, part of Bio-Techne | Catalog # BAF3885

Submit a review

Product Datasheet / COA / SDS

View Available Conjugates

Conjugate

Catalog #

Unconjugated

AF3885

View Product

100% Guarantee

Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry, Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG

View all Lipin 1 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Lipin 1
Asp448-Leu574
Accession # Q14693

Specificity

Detects human Lipin 1 in Western blots. In Western blots, less than 2% cross-reactivity with recombinant human (rh) Lipin 2 and rhLipin 3 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Lipin 1 Biotinylated Antibody

Lipin 1 in SK-Mel-28 Human Cell Line.

Lipin 1 was detected in immersion fixed SK-Mel-28 human malignant melanoma cell line using Goat Anti-Human Lipin 1 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF3885) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; NL999) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human Lipin 1 Biotinylated Antibody

Application

Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed SK-Mel-28 human malignant melanoma cell line

Western Blot

0.1 µg/mL
Sample: Recombinant Human Lipin 1

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lipin 1

Lipin 1 is a 100 kDa phosphatidate phosphatase. Mammalian Lipin 1 has been identified as an enzyme that regulates fat metabolism. There is one human Lipin-1. In mice, two Lipin-1 isoforms have been reported, alpha and beta. Lipin 1 alpha is a nuclear protein and is 96% and 98 % identical to human Lipin 1 and rat Lipin 1 alpha, respectively. Lipin 1 beta is cytoplasmic.

Long Name

Phosphatidate Phosphatase LPIN1

Alternate Names

LPIN1

Entrez Gene IDs

23175 (Human); 14245 (Mouse); 313977 (Rat)

Gene Symbol