Human LPAR1/LPA1/EDG-2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB9963

R&D Systems, part of Bio-Techne
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MAB9963-100
MAB9963-SP
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 999807

View all LPAR1/LPA1/EDG-2 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human LPAR1/LPA1/EDG-2
Met1-Lys50
Accession # Q92633

Specificity

Detects human LPAR1/LPA1/EDG-2 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human LPAR1/LPA1/EDG-2 Antibody

LPAR1/LPA1/EDG-2 antibody in Human Prostate Cancer Tissue by Immunohistochemistry (IHC-P).

LPAR1/LPA1/EDG-2 in Human Prostate Cancer Tissue.

LPAR1/LPA1/EDG-2 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Mouse Anti-Human LPAR1/LPA1/EDG-2 Monoclonal Antibody (Catalog # MAB9963) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surfaces and cytoplasm in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human LPAR1/LPA1/EDG-2 Antibody

Application
Recommended Usage

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human prostate cancer tissue

Reviewed Applications

Read 1 review rated 5 using MAB9963 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LPAR1/LPA1/EDG-2

Lysophosphatidic acid receptor 1, LPAR1, also known as EDG-2, is a G protein-coupled receptor that binds the lipid signaling molecule lysophosphatidic acid (LPA). EDG molecules are G protein-coupled receptors that bind plasma lysophospholipids. The EDG family consists of two subfamilies; the S1P (sphingosine-1-phosphate) subfamily consisting of EDG-1, 3, 5, 6, and 8, and the LPA subfamily that contains EDG-2, 4 and 7. The S1P family regulates essential cellular processes such as proliferation, migration, cytoskeletal organization, and differentiation.

Long Name

Lysophosphatidic Acid Receptor 1

Alternate Names

EDG2, GPCR26, GPR26, LPA1, rec.1.3, vzg-1

Entrez Gene IDs

1902 (Human); 14745 (Mouse)

Gene Symbol

LPAR1

UniProt

Q92633

Additional LPAR1/LPA1/EDG-2 Products

Product Documents for Human LPAR1/LPA1/EDG-2 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human LPAR1/LPA1/EDG-2 Antibody

For research use only

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