Human LPAR1/LPA1/EDG-2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF9963
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
Western Blot
Cited:
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human LPAR-1 CD33 protein
containing the sequences for the four extracellular domains, amino acids (aa) 1-50, aa112-125,
aa190-205 and aa281-294
Accession # Q92633
Accession # Q92633
Specificity
Detects human LPAR-1/LPA1/EDG-2 in direct ELISAs.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human LPAR1/LPA1/EDG-2 Antibody
Detection of Human LPAR1/LPA1/EDG-2 by Western Blot.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line, human brain (cerebellum) tissue, and human liver tissue (negative control). PVDF membrane was probed with 2 µg/mL of Goat Anti-Human LPAR1/LPA1/EDG-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF9963) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for LPAR1/LPA1/EDG-2 at approximately 48-51 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human LPAR1/LPA1/EDG-2 Antibody
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: Jurkat human acute T cell leukemia cell line and Human brain (cerebellum) tissue
Sample: Jurkat human acute T cell leukemia cell line and Human brain (cerebellum) tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LPAR1/LPA1/EDG-2
Lysophosphatidic acid receptor 1, LPAR1, also known as EDG-2, is a G protein-coupled receptor that binds the lipid signaling molecule lysophosphatidic acid (LPA). EDG molecules are G protein-coupled receptors that bind plasma lysophospholipids. The EDG family consists of two subfamilies; the S1P (sphingosine-1-phosphate) subfamily consisting of EDG-1, 3, 5, 6, and 8, and the LPA subfamily that contains EDG-2, 4 and 7. The S1P family regulates essential cellular processes such as proliferation, migration, cytoskeletal organization, and differentiation.
Long Name
Lysophosphatidic Acid Receptor 1
Alternate Names
EDG2, GPCR26, GPR26, LPA1, rec.1.3, vzg-1
Gene Symbol
LPAR1
UniProt
Additional LPAR1/LPA1/EDG-2 Products
Product Documents for Human LPAR1/LPA1/EDG-2 Antibody
Product Specific Notices for Human LPAR1/LPA1/EDG-2 Antibody
For research use only
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