Human LPAR2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6396
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human LPAR2
Met1-Val33, His91-Gly103, Ser168-Ser188, Asp264-Tyr279
Accession # Q9HBW0
Met1-Val33, His91-Gly103, Ser168-Ser188, Asp264-Tyr279
Accession # Q9HBW0
Specificity
Detects human LPAR2 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human LPAR1 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human LPAR2 Antibody
Detection of Human LPAR2 by Western Blot.
Western blot shows lysates of human peripheral blood lymphocytes (PBL). PVDF Membrane was probed with 1 µg/mL of Human LPAR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6396) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for LPAR2 at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.Applications for Human LPAR2 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Human peripheral blood lymphocytes (PBL)
Sample: Human peripheral blood lymphocytes (PBL)
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LPAR2/LPA2/EDG-4
LPAR2 (Lysophosphatidic acid receptor 2; also LPA2 and EDG4) is a 39 kDa member of the GPCR1 family of proteins. It has limited expression, and is found on mammalian macrophages, fibroblasts and cardiac myocytes. LPAR2 binds to, and is activated by LPA, a molecule principally derived from activated platelets. Although LPAR2 appears to prefer myristoyl and palmitoyl LPA, multiple forms of LPA are recognized by the receptor. LPAR2 reportedly mediates chemotaxis, arachidonic acid release, and cell rounding. Human LPAR2 is a 351 amino acid (aa) 7–transmembrane (TM) protein (SwissProt # Q9HBW0). It contains a 33 aa extracellular N-terminus (aa #1-33), seven TM segments (aa #34-297) and a 54 aa C-terminal cytoplasmic tail. There is one splice variant that is associated with tumor cells, and which originally was given the designation EDG4 (endothelium differentiation gene 4). This shows a 35 aa substitution for aa #348-351. Over aa #1-33, 91-103, 168-188 and 264-279 collectively, human LPAR2 shares 75% aa identity with mouse LPAR2.
Long Name
Lysophosphatidic Acid Receptor 2
Alternate Names
EDG4, LPA2, LPAR2
Gene Symbol
LPAR2
UniProt
Additional LPAR2/LPA2/EDG-4 Products
Product Documents for Human LPAR2 Antibody
Product Specific Notices for Human LPAR2 Antibody
For research use only
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