Human LPAR4/LPA4 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB10217
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 561203
Product Specifications
Immunogen
Human embryonic kidney cell line HEK293-derived transfected with human LPAR4/LPA4
Accession # Q99677
Accession # Q99677
Specificity
Detects human LPAR4/LPA4 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human LPAR4/LPA4 Antibody
Detection of LPAR4 in HEK293 Human Cell Line Transfected with Human LPAR4 and eGFP by Flow Cytometry.
HEK293 human embryonic kidney cell line transfected with (A) Human LPAR4 or (B) irrelevant protein, and EGFP, was stained with Mouse Anti-Human LPAR4 Monoclonal Antibody (Catalog # MAB10217) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on Mouse IgG2B isotype control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.Applications for Human LPAR4/LPA4 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: HEK293 Human Cell Line Transfected with Human LPAR4 and eGFP
Sample: HEK293 Human Cell Line Transfected with Human LPAR4 and eGFP
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LPAR4/LPA4
Lysophosphatidic acid receptor 4, also known as LPA4, P2Y purinoceptor 9 (P2RY9) or GPR23, is a protein that in humans is encoded by the LPAR4 gene. LPA4 is a G protein-coupled receptor (GPCR) that binds the lipid signaling molecule lysophosphatidic acid (LPA) and mediates diverse cellular activities. Most LPA receptors share similarities with members of the S1P1/Edg family. GPA4, originally named P2Y9/GPR23, has been described as a fourth LPA receptor, LPA4, that together with LPA5 and LPA6 form a subfamily of LPA receptors structurally distant from the Edg family.
Long Name
Lysophosphatidic Acid Receptor 4
Alternate Names
GPCR23, GPR23, P2RY9, P2Y9
Gene Symbol
LPAR4
UniProt
Additional LPAR4/LPA4 Products
Product Documents for Human LPAR4/LPA4 Antibody
Product Specific Notices for Human LPAR4/LPA4 Antibody
For research use only
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