Human LRH-1/NR5A2 Antibody

R&D Systems, part of Bio-Techne | Catalog # PP-H2325-00

R&D Systems, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Direct ELISA, Immunoprecipitation, Western Blot

Cited:

Chromatin Immunoprecipitation (ChIP), Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Clone # H2325

View all LRH-1/NR5A2 Antibodies »

Product Specifications

Immunogen

Recombinant human LRH-1/NR5A2
aa 161-280

Specificity

This antibody specifically recognizes human LRH-1 alpha and LRH-1 beta and cross-reacts with mouse and rat LRH-1. Not yet tested in other species.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3

Applications for Human LRH-1/NR5A2 Antibody

Application
Recommended Usage

Direct ELISA

This antibody can be used at 2 ng/mL with the appropriate secondary reagents to detect human LRH-1.

Immunoprecipitation

Optimal dilutions should be determined by each laboratory.

Western Blot

This antibody can be used at 1 μg/mL with the appropriate secondary reagents to detect human LRH-1.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

A liquid formulation in physiologic saline with 0.1% NaN3.

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

This antibody is stable for greater than six months when held at -20 °C in a manual defrost freezer or at -70 °C. Upon thawing, the antibody can be stored at 2-8 °C for at least 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Background: LRH-1/NR5A2

Liver Receptor Homologous Protein (LRH-1, FTF, CPF, hB1F, FTZ-F1b; NR5A2) is a member of the Orphan Nuclear Receptor family. LRH-1 is a mammalian homolog of the Drosophila Fushi Tarazu F1 Gene product. LRH-1 is expressed in the liver and intestine. LRH-1 is involved in the regulation of genes which participate in steroid, bile acid and cholesterol homeostasis.

Long Name

Liver Receptor Homolog 1/Nuclear Receptor Subfamily 5, Group A, Member 2

Alternate Names

B1F2, FTZ-F1 beta, LRH1, NR5A2

Entrez Gene IDs

2494 (Human)

Gene Symbol

NR5A2

Additional LRH-1/NR5A2 Products

Product Documents for Human LRH-1/NR5A2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human LRH-1/NR5A2 Antibody

For research use only

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