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Human LRP-11 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF8355

R&D Systems, part of Bio-Techne
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AF8355
AF8355-SP

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

Product Specifications

Immunogen

E. coli-derived recombinant human LRP-11
Ala38-Gly450 (Pro92Arg)
Accession # Q86VZ4

Specificity

Detects human LRP-11 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human LRP-11 Antibody

Detection of Human LRP-11 antibody by Western Blot.

Detection of Human LRP-11 by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line and PANC-1 human pancreatic carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human LRP-11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8355) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for LRP-11 at approximately 60 and 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
LRP-11 antibody in Human Brain by Immunohistochemistry (IHC-P).

LRP-11 in Human Brain.

LRP-11 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Sheep Anti-Human LRP-11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8355) at 0.3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human LRP-11 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cortex)

Western Blot

2 µg/mL
Sample: Jurkat human acute T cell leukemia cell line and PANC‑1 human pancreatic carcinoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LRP-11

LRP11 (Low-density lipoprotein receptor-related protein 11) is a 500 amino acid single-pass type I membrane glycoprotein that exists as 2 alternatively spliced isoforms with predicted molecular weights of 53 and 25 kDa, observed 60 and 35 kDa respectively. LRP11 is a novel member of the LDLR family of proteins. Over aa 38-450, human LRP11 shares 67% aa identity with mouse LRP11.

Long Name

LDL Receptor-related Protein 11

Alternate Names

LRP11, MANSC3

Entrez Gene IDs

84918 (Human); 237253 (Mouse); 292462 (Rat)

Gene Symbol

LRP11

UniProt

Additional LRP-11 Products

Product Documents for Human LRP-11 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human LRP-11 Antibody

For research use only

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