Human LRP-11 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF8355
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human LRP-11
Ala38-Gly450 (Pro92Arg)
Accession # Q86VZ4
Ala38-Gly450 (Pro92Arg)
Accession # Q86VZ4
Specificity
Detects human LRP-11 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human LRP-11 Antibody
Detection of Human LRP-11 by Western Blot.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line and PANC-1 human pancreatic carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human LRP-11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8355) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for LRP-11 at approximately 60 and 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.LRP-11 in Human Brain.
LRP-11 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Sheep Anti-Human LRP-11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8355) at 0.3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human LRP-11 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cortex)
Sample: Immersion fixed paraffin-embedded sections of human brain (cortex)
Western Blot
2 µg/mL
Sample: Jurkat human acute T cell leukemia cell line and PANC‑1 human pancreatic carcinoma cell line
Sample: Jurkat human acute T cell leukemia cell line and PANC‑1 human pancreatic carcinoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LRP-11
LRP11 (Low-density lipoprotein receptor-related protein 11) is a 500 amino acid single-pass type I membrane glycoprotein that exists as 2 alternatively spliced isoforms with predicted molecular weights of 53 and 25 kDa, observed 60 and 35 kDa respectively. LRP11 is a novel member of the LDLR family of proteins. Over aa 38-450, human LRP11 shares 67% aa identity with mouse LRP11.
Long Name
LDL Receptor-related Protein 11
Alternate Names
LRP11, MANSC3
Gene Symbol
LRP11
UniProt
Additional LRP-11 Products
Product Documents for Human LRP-11 Antibody
Product Specific Notices for Human LRP-11 Antibody
For research use only
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