HEK293 cells transfected with Human LRRC-15 and eGFP were stained with eGFP and either (A) Rabbit Anti-Human LRRC15 Monoclonal Antibody (Catalog # MAB11324) or (B) Normal Rabbit IgG Control (Catalog # MAB1050) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). View our protocol for Staining Membrane-associated Proteins.

Detection of Human LRRC15 by Simple Western^TM.

Simple Western lane view shows lysates of U-87 MG human glioblastoma/astrocytoma cell line, loaded at 0.2 mg/mL. A specific band was detected for LRRC15 at approximately 104 kDa (as indicated) using 20 µg/mL of Rabbit Anti-Human LRRC15 Monoclonal Antibody (Catalog # MAB11324). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of Human LRRC15 by Western Blot.

Western blot shows lysates of Human skin and U-118-MG human glioblastoma/astrocytoma cell line. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human LRRC15 Monoclonal Antibody (Catalog # MAB11324) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for LRRC15 at approximately ~70-80kDa kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Applications for Human LRRC15 Antibody

Application

Recommended Usage

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 cells transfected with Human LRRC-15 and eGFP

Simple Western

20 µg/mL
Sample: Lysates of U-87 MG human glioblastoma/astrocytoma cell line

Western Blot

2 µg/mL
Sample: Lysates of Human skin and U‑118‑MG human glioblastoma/astrocytoma cell line

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LRRC15

LRRC15 is a type-I 15-leucine-rich repeat containing membrane protein that is located on chromosome 3 at 3q29. It is a 581 aa membrane protein that belong to the LRR superfamily and lacks an intracellular signaling domain. Reports indicate that it is highly expressed on cancer-associated fibroblasts in numerous solid tumors, and in the epithelial cancer cell compartment of glioblastoma, sarcomas and melanoma tumors. LRRC15 functions by regulating cell-cell and cell-extracellular matrix (ECM) interactions, likely partnering with fibronectin, laminin and collagen IV through its extracellular leucine-rich repeats. LRRC15 is reported in multiple solid tumor types including breast, ovarian and cervical cancers where it exhibits Increased stromal expression (1). Recent studies indicate that LRRC15 is co-expressed with COL10A1, a gene that is overexpressed in many types of solid tumors, including breast cancer (2).

References

Cancer Res. 2022 March 15; 82(6): 1038. doi:10.1158/0008-5472.CAN-21-0622.
Biosci Rep. 2020 Feb 28; 40(2): BSR20193286. doi: 10.1042/BSR20193286.

Long Name

Leucine-rich Repeat Containing 15

Alternate Names

LIB

Entrez Gene IDs

131578 (Human); 74488 (Mouse); 246296 (Rat)

Gene Symbol

LRRC15

UniProt

Q8TF66

Additional LRRC15 Products

Product Documents for Human LRRC15 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human LRRC15 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human LRRC15 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB11324

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all LRRC15 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human LRRC15 Antibody

Detection of LRRC15 in HEK293 cells by Flow Cytometry.

Detection of Human LRRC15 by Simple WesternTM.