Human LRRTM4 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5377
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human LRRTM4
Gln31-Lys424
Accession # Q86VH4
Gln31-Lys424
Accession # Q86VH4
Specificity
Detects human LRRTM4 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross‑reactivity with recombinant human (rh) LRRTM1, rhLRRTM2, and rhLRRTM3 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human LRRTM4 Antibody
Detection of Human LRRTM4 by Western Blot.
Western blot shows lysates of human brain tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human LRRTM4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5377) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for LRRTM4 at approximately 65 and 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.Applications for Human LRRTM4 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Human brain tissue
Sample: Human brain tissue
Reviewed Applications
Read 1 review rated 4 using AF5377 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LRRTM4
LRRTM4 (leucine rich repeat transmembrane protein 4) is a 59kDa (predicted) and 67 kDa (predicted) glycoprotein, member of the LRRTM family of proteins. All members of this family are type I transmembrane proteins that contain 10 external LRR repeats plus a short cytoplasmic tail that binds PDF motifs. LRRTM4 is found in the neurons of the cerebral cortex, dentate gyrus granule cells, and cerebellar Purkinje cells. Mature human LRRTM4 is 560 amino acids (aa) in length. The extracellular region contains one Cys‑rich N-terminal flanking domain (aa 33‑59), multiple LRRs (aa 60‑299), and a Cys‑rich C-terminal flanking domain (aa 306‑363). There is one splice variant that shows a Val substitution for cytoplasmic aa 518‑590. Over aa 31‑424, human LRRTM4 shares 95% aa identity with mouse LRRTM4.
Long Name
Leucine Rich Repeat Transmembrane Neuronal 4
Alternate Names
FLJ12568, leucine rich repeat transmembrane neuronal 4, leucine-rich repeat transmembrane neuronal 4 protein, leucine-rich repeat transmembrane neuronal protein 4, MGC120633, MGC120636
Gene Symbol
LRRTM4
UniProt
Additional LRRTM4 Products
Product Documents for Human LRRTM4 Antibody
Product Specific Notices for Human LRRTM4 Antibody
For research use only
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