Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 71319

View all Lymphotoxin beta R/TNFRSF3 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Lymphotoxin betaR/TNFRSF3
Gln31-Met227
Accession # P36941

Specificity

Detects human Lymphotoxin betaR/TNFRSF3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) 4-1BB, rhBAFF R, rhCD27, rhCD30, rhCD40, rhDR3, rhDR6, rhEDAR, rhFas, rhGITR, rhHVEM, recombinant mouse (rm) Lymphotoxin  betaR, rhNGF R, rhOPG, rhRELT, rhTAJ, rhTNF RI or rhTNF RII is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Lymphotoxin  betaR/TNFRSF3 APC-conjugated Antibody

Detection of Lymphotoxin betaR/TNFRSF3 antibody in Human PBMCs antibody by Flow Cytometry.

Detection of Lymphotoxin betaR/TNFRSF3 in Human PBMCs by Flow Cytometry.

Human peripheral blood monocytes (PBMCs) were stained with Mouse Anti-Human Lymphotoxin betaR/TNFRSF3 APC-conjugated Monoclonal Antibody (Catalog # FAB629A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Detection of Lymphotoxin  betaR/TNFRSF3 in A549 cells by Flow Cytometry.

A549 cells were stained with Mouse Anti-Human Lymphotoxin  betaR/TNFRSF3 APC-conjugated Monoclonal Antibody (Catalog # FAB629A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Lymphotoxin  betaR/TNFRSF3 APC-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Human peripheral blood monocytes (PBMCs) and A549 human lung carcinoma cell line. 

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: Lymphotoxin beta R/TNFRSF3

Lymphotoxin beta receptor (LT betaR), also known as TNF RIII and TNF R-related protein (TNF Rrp) is a member of the TNF receptor superfamily, designated TNFRSF3. Human LT betaR cDNA encodes a 435 amino acid (aa) residue type I membrane protein with a putative 30 aa residue signal peptide, a 193 aa residue extracellular domain and a 171 aa residue cytoplasmic domain. The extracellular domain of LT betaR contains four cysteine-rich motifs characteristic of the TNF receptor superfamily. The cytoplasmic region of LT betaR shares little sequence similarity with other TNF receptor family members, suggesting that different signaling mechanisms may be used. LT betaR is expressed in a variety of tissues including visceral and lymphoid tissues. LT betaR is also expressed by cell lines of monocytic, epithelial, and fibroblastic origins but not by T and B lymphocytes. Human and mouse LT betaR share 76% aa sequence homology. The TNF family ligands that have been shown to bind and activate LT betaR include LIGHT (also a ligand for HVEM) and the heterotrimeric lymphotoxin LT alpha1/ beta2 or LT alpha2/ beta1. Depending on the cell type, activation of LT betaR has been shown to induce NF kappaB activation, chemokine production, growth arrest, and apoptosis. In vivo, LT betaR has been shown to play a critical role in controlling cellular immune functions and lymphoid organogenesis.

References

  1. Zhai, Y. et al. (1998) J. Clin. Invest. 102:1142.
  2. Rennert, P.D. et al. (1998) Immunity 9:71.
  3. Degli-Esposti, M.A. et al. (1997) J. Immunol 158:1756.
  4. Mackay, F. et al. (1996) J. Biol. Chem. 271:8618.
  5. Crowe, P.D. et al. (1994) Science 264:707.

Long Name

Lymphotoxin beta Receptor

Alternate Names

LTBR, LymphotoxinbR, TNF RIII, TNF Rrp, TNFRSF3

Entrez Gene IDs

4055 (Human); 17000 (Mouse); 297604 (Rat); 102135920 (Cynomolgus Monkey); 712550 (Rhesus Macaque)

Gene Symbol

LTBR

UniProt

P36941

Additional Lymphotoxin beta R/TNFRSF3 Products

Product Documents for Human Lymphotoxin  betaR/TNFRSF3 APC-conjugated Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Lymphotoxin  betaR/TNFRSF3 APC-conjugated Antibody

For research use only

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