HEK293 human embryonic kidney cell line transfected with either (A) human LYPD8 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human LYPD8 Monoclonal Antibody (Catalog # MAB9087) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins.

LYPD8 in HEK293 Human Cell Line.

LYPD8 was detected in immersion fixed HEK293 human embryonic kidney cell line using Mouse Anti-Human LYPD8 Monoclonal Antibody (Catalog # MAB9087) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell membrane and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human LYPD8 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 human embryonic kidney cell line transfected with human LYPD8 and eGFP

Immunocytochemistry

5-25 µg/mL
Sample: Immersion fixed HEK293 human embryonic kidney cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LYPD8

Ly6/PLAUR domain containing 8 (LYPD8), is a GPI-linked protein with structural similarity to the urokinase-type plasminogen activator receptor (uPAR) (1). Mature human LYPD8 contains one uPAR/Ly6 domain and a Ser/Thr/Pro-rich (STP) region that may serve as a target for protease mediated shedding as has been shown for the related C4.4A/LYPD3 molecule (2, 3). Mature human LYPD8 shares 40% amino acid sequence identity with mouse and rat LYPD8.

References

Kong, H.K. and J.H. Park (2012) BMB Rep. 45:595.
Hansen, L.V. et al. (2004) Biochem. J. 380:845.
Esselens, C.W. et al. (2008) Biol. Chem. 389:1075.

Long Name

Ly6/PLAUR Domain-containing Protein 8

Entrez Gene IDs

646627 (Human); 70163 (Mouse); 691259 (Rat)

Gene Symbol

LYPD8

UniProt

Q6UX82

Additional LYPD8 Products

Product Documents for Human LYPD8 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

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Product Specific Notices for Human LYPD8 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human LYPD8 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB9087

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all LYPD8 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human LYPD8 Antibody

Detection of LYPD8 in HEK293 Human Cell Line Transfected with Human LYPD8 and eGFP by Flow Cytometry.