Human Lysine (K)-specific Demethylase 6B/KDM6B Antibody
R&D Systems, part of Bio-Techne | Catalog # AF7300
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Lysine (K)-specific Demethylase 6B/KDM6B (isoform 1)
Pro1530-Arg1682
Accession # NP_001073893
Pro1530-Arg1682
Accession # NP_001073893
Specificity
Detects human Lysine (K)-specific Demethylase 6B/KDM6B in direct ELISAs.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human Lysine (K)-specific Demethylase 6B/KDM6B Antibody
Lysine (K)-specific Demethylase 6B/KDM6B in BG01V Human Stem Cells.
Lysine (K)-specific Demethylase 6B/KDM6B was detected in immersion fixed BG01V human embryonic stem cells, differentiated (upper panel) and undifferentiated (lower panel) using Goat Anti-Human Lysine (K)-specific Demethylase 6B/KDM6B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7300) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human Lysine (K)-specific Demethylase 6B/KDM6B Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed BG01V human embryonic stem cells, differentiated and undifferentiated
Sample: Immersion fixed BG01V human embryonic stem cells, differentiated and undifferentiated
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Lysine (K)-specific Demethylase 6B/KDM6B
KDM6B (Lysine [K]-specific demethylase 6B; also JMJD3) is a 170-185 kDa member of the UTX family of enzymes. It is expressed in both embryo and adult, and specifically demethylates tri- and di-methylated Lys27 on histone H3 (H3K27). Methylation of H3K27 by PRC2 inactivates genes, while demethylation of methylated H3K27 by KDM6B activates genes. Human KDM6B is 1643 amino acids (aa) in length. It contains two Pro-rich regions (aa 32-85 and 1046-1082) plus one jumonji domain (aa 1339-1502). One potential splice variant is reported that contains a 39 aa insertion after Leu1636. Over aa 1530-1682, which is a sequence that contains the 39 aa insertion, human KDM6B shares 75% aa sequence identity with mouse KDM6B. When the insertion is disregarded, human and mouse KDM6B are identical in aa sequence over the above range of amino acids.
Alternate Names
JMJD3, KDM6B, Lysine (K)specific Demethylase 6B
Gene Symbol
KDM6B
UniProt
Additional Lysine (K)-specific Demethylase 6B/KDM6B Products
Product Documents for Human Lysine (K)-specific Demethylase 6B/KDM6B Antibody
Product Specific Notices for Human Lysine (K)-specific Demethylase 6B/KDM6B Antibody
For research use only
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