Human Lysosomal alpha-Glucosidase Antibody

Detection of Human Lysosomal alpha‑Glucosidase by Western Blot.

Western blot shows lysates of T47D human breast cancer cell line and human peripheral blood mononuclear cells (PBMC). PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human Lysosomal a-Glucosidase Monoclonal Antibody (Catalog # MAB83291) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for Lysosomal a-Glucosidase at approximately 75 kDa and 105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Lysosomal alpha‑Glucosidase in Human Kidney.

Lysosomal a-Glucosidase was detected in immersion fixed paraffin-embedded sections of human kidney using Rabbit Anti-Human Lysosomal a-Glucosidase Monoclonal Antibody (Catalog # MAB83291) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.