Human macrophages treated with LPS and Recombinant Human TNF-a (210-TA) were stained with Mouse Anti-Human M-CSF PE-conjugated Monoclonal Antibody (Catalog # IC2161P, filled histogram) or isotype control antibody (IC003P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). View our protocol for Staining Intracellular Molecules.

Detection of M-CSF in MG-63 cells by Flow Cytometry.

MG-63 cells were stained with Mouse Anti-Human M-CSF PE-conjugated Monoclonal Antibody (Catalog # IC2161P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Applications for Human M-CSF PE-conjugated Antibody

Application

Recommended Usage

Intracellular Staining by Flow Cytometry

0.25 µL/10⁶ cells
Sample: Human macrophages treated with LPS and Recombinant Human TNF-alpha (Catalog # 210-TA) fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005) and MG-63 human osteosarcoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: M-CSF

M-CSF, also known as CSF-1, is produced by a variety of cell types in either a membrane-anchored or secreted soluble form. It is a glycosylated, disulfide-linked homodimer that binds to the M-CSF receptor expressed by cells of the monocyte-macrophage lineage.

Long Name

Macrophage Colony Stimulating Factor

Alternate Names

CSF-1, CSF1, Lanimostim, MCSF

Entrez Gene IDs

1435 (Human); 12977 (Mouse)

Gene Symbol

CSF1

Additional M-CSF Products

Product Documents for Human M-CSF PE-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human M-CSF PE-conjugated Antibody

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

Human M-CSF PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # IC2161P

Key Product Details

Validated by

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all M-CSF Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human M-CSF PE-conjugated Antibody

Detection of M‑CSF in Human Macrophages by Flow Cytometry.