Human MARCO Antibody
R&D Systems, part of Bio-Techne | Catalog # AF7586
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Flow Cytometry
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human MARCO
Met79-Val520
Accession # Q9UEW3
Met79-Val520
Accession # Q9UEW3
Specificity
Detects human MARCO in direct ELISAs and Western blots. In direct ELISA, approximately 10% cross-reactivity with recombinant mouse MARCO is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human MARCO Antibody
Detection of Human MARCO by Western Blot.
Western blot shows lysates of THP-1 human acute monocytic leukemia cell line, human lung tissue, and human liver tissue untreated (-) or treated (+) with 80 nM PMA for 72 hours. PVDF membrane was probed with 0.25 µg/mL of Sheep Anti-Human MARCO Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7586) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for MARCO at approximately 65-75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human MARCO Antibody
Application
Recommended Usage
Western Blot
0.25 µg/mL
Sample: THP‑1 human acute monocytic leukemia cell line, treated with PMA, human lung tissue, and human liver tissue
Sample: THP‑1 human acute monocytic leukemia cell line, treated with PMA, human lung tissue, and human liver tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MARCO
MARCO (Macrophage receptor with collagenous structure; also scavenger receptor class A member 2/SR-AII) is a 65-70 kDa pattern recognition receptor/PRR that belongs to class A of the SR family, SRCR superfamily of molecules. It is expressed by macrophages, monocytes and Kupffer cells, and apparently binds to a number of microbes, including Gram+ bacteria (Staph and Strep) and Gram- bacteria (Neisseria and coliforms), plus crystalline silica. Human MARCO is a 520 amino acid (aa) type II tranamembrane glycoprotein. It contains a 43 aa cytoplasmic region and a 456 aa extracellular domain/ECD (aa 65-520). The ECD possesses one collagen-like region (aa 147-419) and an SRCR domain (aa 424-519). Human MARCO is believed to form trimers and/or multimers, and these are likely noncovalent associations. Over aa 79-520, human and mouse MARCO share 70% aa sequence identity.
Long Name
Macrophage Receptor with Collagenous Structure/Scavenger Receptor, Class A, Member 2
Alternate Names
SCARA2
Gene Symbol
MARCO
UniProt
Additional MARCO Products
Product Documents for Human MARCO Antibody
Product Specific Notices for Human MARCO Antibody
For research use only
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