Human MBL Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2307
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Bacteria - Moraxella catarrhalis
Applications
Validated:
ELISA Capture (Matched Antibody Pair), Western Blot
Cited:
ELISA Development, Flow Cytometry, Neutralization, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human MBL
Glu21-Ile248
Accession # AAH96182
Glu21-Ile248
Accession # AAH96182
Specificity
Detects human MBL in ELISAs and Western blots. In sandwich ELISAs, less than 0.05% cross-reactivity with recombinant mouse (rm) MBL and rmMBL‑2 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human MBL Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Human MBL (Catalog # 2307-MB)
Sample: Recombinant Human MBL (Catalog # 2307-MB)
Human MBL Sandwich Immunoassay
ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 0.2-0.8 µg/mL
Use in combination with these reagents:
Use in combination with these reagents:
- Detection Reagent: Human MBL Biotinylated Antibody (Catalog # BAF2307)
- Standard: Recombinant Human MBL Protein (Catalog # 2307-MB)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 3 reviews rated 4.3 using AF2307 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MBL
MBL belongs to the collectin family of innate immune defense proteins, which bind to an array of carbohydrate patterns on pathogen surfaces. MBL homotrimerizes to form a structural unit that further associates into oligomeric structures. Circulating MBL is complexed with three MBL-associated serine proteases (MASPs). Human MBL shares approximately 60% amino acid sequence identity with mouse MBL1 or MBL2.
References
Long Name
Mannose Binding Lectin
Alternate Names
COLEC1, Collectin-1, MBL2, MBL2D, MBP1
Entrez Gene IDs
4153 (Human)
Gene Symbol
MBL2
UniProt
Additional MBL Products
Product Documents for Human MBL Antibody
Product Specific Notices for Human MBL Antibody
For research use only
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