Human MCAM/CD146 Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF932
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Block/Neutralize
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human MCAM/CD146
Met1-Gly559
Accession # AAA20824
Met1-Gly559
Accession # AAA20824
Specificity
Detects human MCAM/CD146 in Western blots. In Western blots, less than 5% cross-reactivity with recombinant human (rh) ALCAM, recombinant mouse (rm) MAdCAM-1, and rmOCAM is observed and less than 1% cross-reactivity with rhBCAM, rhEpCAM, rhNCAM-L1, and rhTROP-2 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human MCAM/CD146 Biotinylated Antibody
MCAM/CD146 in Human Melanoma.
MCAM/CD146 was detected in immersion fixed paraffin-embedded sections of human melanoma tissue using Goat Anti-Human MCAM/CD146 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF932) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human MCAM/CD146 Biotinylated Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human normal breast and human skin cancer tissue
Sample: Immersion fixed paraffin-embedded sections of human normal breast and human skin cancer tissue
Western Blot
0.1 µg/mL
Sample: Recombinant Human MCAM/CD146
Sample: Recombinant Human MCAM/CD146
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MCAM/CD146
Long Name
Melanoma Cell Adhesion Molecule
Alternate Names
CD146, L-Gicerin, MUC18
Gene Symbol
MCAM
UniProt
Additional MCAM/CD146 Products
Product Documents for Human MCAM/CD146 Biotinylated Antibody
Product Specific Notices for Human MCAM/CD146 Biotinylated Antibody
For research use only
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