Human Mcl-1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB8281

R&D Systems, part of Bio-Techne
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MAB8281
MAB8281-SP
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 602901

View all Mcl-1 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Mcl-1
Met231-Arg350
Accession # Q07820

Specificity

Detects human Mcl-1 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Mcl-1 Antibody

Mcl-1 antibody in Human Lymphoma by Immunohistochemistry (IHC-P).

Mcl‑1 in Human Lymphoma.

Mcl-1 was detected in immersion fixed paraffin-embedded sections of human lymphoma using Mouse Anti-Human Mcl-1 Monoclonal Antibody (Catalog # MAB8281) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). Specific staining was localized to the cytoplasm and plasma membranes of lymphocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Mcl-1 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human lymphoma
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Mcl-1

Mcl-1 (myeloid cell leukemia-1) is a member of the Bcl-2 family of proteins. Alternative splicing creates two distinct isoforms. The 40 kDa, 350 amino acid (aa) isoform 1, also known as Mcl-1L (long), enhances cell survival by inhibiting apoptosis, while the ~35 kDa, 271 aa isoform 2, also known as Mcl-1S (short; with divergence in the last 41 aa) promotes apoptosis. The elimination of Mcl-1L is a required step for DNA damage-induced apoptosis. Mcl-1 can be modified by phosphorylation on S121 and T163 by JNK, which triggers apoptosis, or polyubiquitination, which enhances degradation. Within aa 231-350 (unique to Mcl-1L), human Mcl-1 shares 93% and 96% aa identity with mouse and rat Mcl-1, respectively.

Long Name

Myeloid Cell Leukemia Sequence 1

Alternate Names

BCL2L3, Mcl1

Entrez Gene IDs

4170 (Human); 17210 (Mouse)

Gene Symbol

MCL1

UniProt

Q07820 (Human)

Additional Mcl-1 Products

Product Documents for Human Mcl-1 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Mcl-1 Antibody

For research use only

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