Human MDL-1/CLEC5A APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB2384A

R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 283834

View all MDL-1/CLEC5A Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human MDL‑1/CLEC5A
Tyr26-Lys188
Accession # Q9NY25

Specificity

Detects human MDL‑1/CLEC5A in Western blots. Does not cross-react with recombinant mouse MDL‑1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human MDL-1/CLEC5A APC-conjugated Antibody

Detection of MDL-1/CLEC5A antibody in Human Blood Granulocytes antibody by Flow Cytometry.

Detection of MDL‑1/CLEC5A in Human Blood Granulocytes by Flow Cytometry.

Human peripheral blood granulocytes were stained with (A) Mouse Anti-Human Siglec-3/CD33 PE-conjugated Monoclonal Antibody (Catalog # FAB1137P) and (B) Mouse Anti-Human MDL-1/CLEC5A APC-conjugated Monoclonal Antibody (Catalog # FAB2384A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human MDL-1/CLEC5A APC-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Human peripheral blood granulocytes

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: MDL-1/CLEC5A

MDL-1 is an approximately 40 kDa transmembrane glycoprotein belonging to the C-type lectin superfamily. MDL-1 is expressed on immature myeloid cells, monocytes, macrophages, dendritic cells, neutrophils, NK cells, and osteocytes. It contains a charged lysine in the transmembrane region that enables it to associate with DAP12 and deliver an activating signal. MDL-1 mediates inflammatory responses during autoimmune arthritis and upon binding to Dengue and Japanese encephalitis viruses. The extracellular domain (ECD) of MDL-1 contains a juxtamembrane stalk region and one C-type lectin domain. Within the ECD, human and mouse MDL-1 share 67% amino acid sequence identity.

Long Name

Myeloid DAP12-associating Lectin

Alternate Names

CLEC5A, CLECSF5, MDL1

Entrez Gene IDs

23601 (Human); 23845 (Mouse)

Gene Symbol

CLEC5A

UniProt

Q9NY25

Additional MDL-1/CLEC5A Products

Product Documents for Human MDL-1/CLEC5A APC-conjugated Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human MDL-1/CLEC5A APC-conjugated Antibody

For research use only

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