Human MEGF9 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF7768
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Mouse, Rat
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Bioassay
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human MEGF9
Ala36-Asn514
Accession # Q9H1U4
Ala36-Asn514
Accession # Q9H1U4
Specificity
Detects human MEGF9 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human MEGF9 Antibody
Detection of Human MEGF9 by Western Blot.
Western blot shows lysates of IMR-32 human neuroblastoma cell line and human brain (cortex) tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human MEGF9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7768) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for MEGF9 at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
MEGF9 in Human Brain.
MEGF9 was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using Sheep Anti-Human MEGF9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7768) at 1 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counter-stained with hematoxylin (blue). Specific staining was localized to Purkinje neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human MEGF9 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cerebellum)
Sample: Immersion fixed paraffin-embedded sections of human brain (cerebellum)
Western Blot
1 µg/mL
Sample: IMR‑32 human neuroblastoma cell line and human brain (cortex) tissue
Sample: IMR‑32 human neuroblastoma cell line and human brain (cortex) tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MEGF9
MEGF9 (Multiple EGF‑like domains protein 9; also EGF‑like protein 5) is a 63 kDa (predicted) novel transmembrane glycoprotein that shares some homology to beta‑chains of laminin. It is expressed by hepatocytes, cerebellar Purkinje cells, Schwann cells, keratinocytes and intestinal epithelium. MEGF9 is suggested to participate in cell motility, and its absence correlates with tumor cell migration. Mature human MEGF9 is 572 amino acids (aa) in length. It is a single span type I transmembrane protein that contains a 484 aa extracellular region (aa 31‑514) plus a 68 aa C‑terminal cytoplasmic domain. The extracellular region possesses a lengthy Pro‑rich region (aa 55‑200), followed by five EGF‑like domains (aa 204‑451). MEGF9 may run at approximately 160 kDa in SDS‑PAGE, suggesting either heavy glycosylation or dimerization. Over aa 36‑514, human MEGF9 shares 76% aa sequence identity with mouse MEGF9.
Long Name
Multiple EGF-like-domains 9
Alternate Names
EGFL5
Gene Symbol
MEGF9
UniProt
Additional MEGF9 Products
Product Documents for Human MEGF9 Antibody
Product Specific Notices for Human MEGF9 Antibody
For research use only
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