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Human Melanotransferrin/CD228 Alexa Fluor® 488-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB81751G

R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 893416

Product Specifications

Immunogen

Human embryonic kidney cell line HEK293-derived recombinant human Melanotransferrin/CD228
Met1-Gly711
Accession # P08582

Specificity

Detects human Melanotransferrin/CD228 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images

Detection of Melanotransferrin/CD228 antibody in A431 Human Cell Line antibody by Flow Cytometry.

Detection of Melanotransferrin/CD228 in A431 Human Cell Line by Flow Cytometry.

A431 human epithelial carcinoma cell line was stained with Mouse Anti-Human Melanotransferrin/CD228 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB81751G, filled histogram) or isotype control antibody (Catalog # IC002G, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications

Application
Recommended Usage

Flow Cytometry

0.25-1 µg/106 cells
Sample: A431 human epithelial carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Melanotransferrin/CD228

Melanotransferrin, also known as MTF, CD228, Melanoma-associated Antigen p97, MAP97 and MFI2, is a 90-97 kDa sialoglycoprotein member of the transferrin family. Unlike other transferrins, which are secreted, MTF is usually found tethered to the cell membrane by a glycosyl phosphatidyl inositol anchor, with only small amounts of soluble protein detected. MTF is highly expressed on melanoma cells, and at lower levels in salivary gland, pancreas, kidney and testis. Like other transferrins, MTF is an iron-binding protein, and may play roles in cellular proliferation, tumorigenesis, metastasis and migration. Full-length human, mouse and rat MTF is synthesized as a 738 amino acid (aa) protein. Over aa 1-711, human MTF shares 85 and 86% aa identity with mouse and rat MTF, respectively.

Alternate Names

CD228, MAP97, MFI2, MTF, p97

Entrez Gene IDs

4241 (Human); 30060 (Mouse); 288038 (Rat)

Gene Symbol

MELTF

UniProt

Additional Melanotransferrin/CD228 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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