Human Mena Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5304
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Mena
Asn386-Arg513
Accession # Q8N8S7
Asn386-Arg513
Accession # Q8N8S7
Specificity
Detects endogenous human Mena in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human Mena Antibody
Detection of Human Mena by Western Blot.
Western blot shows lysates of MCF-7 human breast cancer cell line, MDA-MB-453 human breast cancer cell line, HUVEC human umbilical vein endothelial cells, and T47D human breast cancer cell line. PVDF membrane was probed with 0.2 µg/mL of Human Mena Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5304) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for Mena at approximately 80 and 88 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.Applications for Human Mena Antibody
Application
Recommended Usage
Western Blot
0.2 µg/mL
Sample: MCF-7 human breast cancer cell line, MDA-MB-453 human breast cancer cell line, HUVEC human umbilical vein endothelial cells, and T47D human breast cancer cell line
Sample: MCF-7 human breast cancer cell line, MDA-MB-453 human breast cancer cell line, HUVEC human umbilical vein endothelial cells, and T47D human breast cancer cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Mena
Mena (mammalian ENA; also protein enabled homolog) is a cytosolic member of the Ena/VASP family of proteins. It runs anomalously high in SDS-PAGE due to a high proline content. Multiple cell types produce Mena, including fibroblasts, myoepithelial cells, neurons and endothelium. Mena promotes the organization of actin filaments in filopodia, and strengthens cell-cell interactions in epithelia. Human Mena is 591 amino acids (aa) in length. It contains one WH1 domain (aa 1‑111), two coiled-coil regions (aa 135‑265 and 557‑587), a Pro-rich segment (aa 310‑373) and an EVH domain (aa 391‑588) that binds G- and F-actin, and mediates tetramerization. There is a 140 kDa neuronal isoform that shows a large 228 aa insertion after Ala268.
Alternate Names
ENAH, NDPP1
Gene Symbol
ENAH
UniProt
Additional Mena Products
Product Documents for Human Mena Antibody
Product Specific Notices for Human Mena Antibody
For research use only
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