Human MESDC2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB5577
Key Product Details
Species Reactivity
Validated:
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 513802
Product Summary for Human MESDC2 Antibody
Immunogen
E. coli-derived recombinant human MESDC2
Ala34-Leu234
Accession # Q14696
Ala34-Leu234
Accession # Q14696
Specificity
Detects human MESDC2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse MESDC2 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human MESDC2 Antibody
Detection of Human MESDC2 by Western Blot.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line and HeLa human cervical epithelial carcinoma cell line. PVDF Membrane was probed with 2 µg/mL of Human MESDC2 Monoclonal Antibody (Catalog # MAB5577) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for MESDC2 at approximately 29 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human MESDC2 Antibody
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: Jurkat human acute T cell leukemia cell line and HeLa human cervical epithelial carcinoma cell line
Sample: Jurkat human acute T cell leukemia cell line and HeLa human cervical epithelial carcinoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MESDC2
Mesoderm development candidate gene 2 (MESDC2), also known as Boca in drosophila, is a 22 kDa protein that is required for formation of the primitive streak and mesoderm during embryogenesis. MESDC2 binds to the Wnt co-receptors LRP5 and LRP6 in the endoplasmic reticulum and is required for their proper folding and cell surface expression. Within aa 34‑234, human MESDC2 shares 87% aa sequence identity with mouse and rat MESDC2, respectively.
Long Name
Mesoderm Development Candidate 2
Alternate Names
BOCA
Entrez Gene IDs
23184 (Human)
Gene Symbol
MESD
UniProt
Additional MESDC2 Products
Product Documents for Human MESDC2 Antibody
Product Specific Notices for Human MESDC2 Antibody
For research use only
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