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Human MESDC2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB5577

R&D Systems, part of Bio-Techne
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MAB5577
MAB5577-SP

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 513802

Product Specifications

Immunogen

E. coli-derived recombinant human MESDC2
Ala34-Leu234
Accession # Q14696

Specificity

Detects human MESDC2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse MESDC2 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human MESDC2 Antibody

Detection of Human MESDC2 antibody by Western Blot.

Detection of Human MESDC2 by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line and HeLa human cervical epithelial carcinoma cell line. PVDF Membrane was probed with 2 µg/mL of Human MESDC2 Monoclonal Antibody (Catalog # MAB5577) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for MESDC2 at approximately 29 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human MESDC2 Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: Jurkat human acute T cell leukemia cell line and HeLa human cervical epithelial carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MESDC2

Mesoderm development candidate gene 2 (MESDC2), also known as Boca in drosophila, is a 22 kDa protein that is required for formation of the primitive streak and mesoderm during embryogenesis. MESDC2 binds to the Wnt co-receptors LRP5 and LRP6 in the endoplasmic reticulum and is required for their proper folding and cell surface expression. Within aa 34‑234, human MESDC2 shares 87% aa sequence identity with mouse and rat MESDC2, respectively.

Long Name

Mesoderm Development Candidate 2

Alternate Names

BOCA

Entrez Gene IDs

23184 (Human)

Gene Symbol

MESD

UniProt

Additional MESDC2 Products

Product Documents for Human MESDC2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human MESDC2 Antibody

For research use only

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