Human Mesothelin Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB32653
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Specifications
Immunogen
Glu296-Asp580
Accession # AAH09272
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human Mesothelin Antibody
Detection of Human Mesothelin by Simple WesternTM.
Simple Western lane view shows lysates of human ovarian cancer tissue and human lung tissue, loaded at 0.2 mg/mL. A specific band was detected for Mesothelin at approximately 65 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human Mesothelin Monoclonal Antibody (Catalog # MAB32653). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.*Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.Detection of Human Mesothelin by Western Blot.
Western blot shows lysates of human ovarian cancer tissue. PVDF Membrane was probed with 2 µg/mL of Human Mesothelin Monoclonal Antibody (Catalog # MAB32653) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Mesothelin at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Human Mesothelin ELISA Standard Curve.
Recombinant Human Mesothelin protein was serially diluted 2-fold and captured by Mouse Anti-Human Mesothelin Monoclonal Antibody (Catalog # MAB32653) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Mesothelin Monoclonal Antibody (Catalog # MAB32654) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).Applications for Human Mesothelin Antibody
ELISA
This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human Mesothelin Monoclonal Antibody (Catalog # MAB32654).
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Mesothelin DuoSet ELISA Kit (Catalog # DY3265) for convenient development of a sandwich ELISA or the Human Mesothelin Quantikine ELISA Kit (Catalog # DMSLN0) for a complete optimized ELISA.
Simple Western
Sample: Human ovarian cancer tissue and human lung tissue
Western Blot
Sample: Human ovarian cancer tissue
Reviewed Applications
Read 1 review rated 3 using MAB32653 in the following applications:
Formulation, Preparation, and Storage
Purification
Reconstitution
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Mesothelin
References
- Hassan, R. et al. (2004) Clin. Cancer Res. 10:3937.
- Kojima, T. et al. (1995) J. Biol. Chem. 270:21984.
- Chang, K. and I. Pastan (1996) Proc. Natl. Acad. Sci. 93:136.
- Onda, M. et al. (2006) Clin. Cancer Res. 12:4225.
- Yamaguchi, N. et al. (1994) J. Biol. Chem. 269:805.
- Muminova, Z.E. et al. (2004) BMC Cancer 4:19.
- Hou, L.-Q. et al. (2008) Develop. Growth Differ. 50:531.
- Ordonez, N.G. (2003) Mod. Pathol. 16:192.
- Argani, P. et al. (2001) Clin. Cancer Res. 7:3862.
- Li, M. et al. (2008) Mol. Cancer Ther. 7:286.
- Scholler, N. et al. (1999) Proc. Natl. Acad. Sci. 96:11531.
- Uehara, N. et al. (2008) Mol. Cancer Res. 6:186.
- Rump, A. et al. (2004) J. Biol. Chem. 279:9190.
- Ho, M. and M.O. Lively (2006) Cancer Epidemiol. Biomarkers Prev. 15:1751.
- Robinson, B.W.S. et al. (2003) Lancet 362:1612.
Alternate Names
Gene Symbol
UniProt
Additional Mesothelin Products
Product Documents for Human Mesothelin Antibody
Product Specific Notices for Human Mesothelin Antibody
For research use only