Human Mesothelin Antibody

R&D Systems, part of Bio-Techne | Catalog # AF3265

R&D Systems, part of Bio-Techne
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AF3265
AF3265-SP
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Conjugate
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Biotin
BAF3265
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all Mesothelin Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Mesothelin isoform 2
Glu296-Gly580
Accession # ABW03459

Specificity

Detects human Mesothelin in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Mesothelin Antibody

Detection of Human Mesothelin antibody by Western Blot.

Detection of Human Mesothelin by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human Mesothelin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3265) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Mesothelin at approximately 69 kDa (as indicated). This experiment was conducted under reducing conditions and Immunoblot Buffer Group 8.

Applications for Human Mesothelin Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Mesothelin

Mesothelin is a 40 kDa, 292 amino acid (aa) GPI-linked glycoprotein found on normal mesothelial cells. It is also highly expressed in pancreatic, gastric and ovarian carcinomas. Mesothelin is synthesized as a preproprotein with a carboxy-terminal hydrophobic proregion that is removed when a GPI-anchor is added. The N-terminal prosegment is cleaved by furin to release a 33 kDa soluble protein that is also known as megakaryocyte potentiating factor (MPF). The membrane bound portion of Mesothelin can also be shed and is detectable in sera. Within the region used as the immunogen, human Mesothelin shares approximately 58% amino acid sequence identity with the mouse or rat protein.

Alternate Names

CAK1, MPF, MSLN, SMR

Entrez Gene IDs

10232 (Human); 56047 (Mouse); 60333 (Rat); 102130460 (Cynomolgus Monkey)

Gene Symbol

MSLN

UniProt

ABW03459

Additional Mesothelin Products

Product Documents for Human Mesothelin Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Mesothelin Antibody

For research use only

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