Human Mesothelin MAb (Clone 420425)
R&D Systems, part of Bio-Techne | Catalog # MAB32655
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 420425
Product Summary for Human Mesothelin MAb (Clone 420425)
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Mesothelin
Glu296-Gly580
Accession # AAH09272
Glu296-Gly580
Accession # AAH09272
Specificity
Detects human Mesothelin in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human Mesothelin MAb (Clone 420425)
Human Mesothelin ELISA Standard Curve.
Recombinant Human Mesothelin protein was serially diluted 2-fold and captured by Mouse Anti-Human Mesothelin Monoclonal Antibody (Catalog # MAB32655) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Human Mesothelin Monoclonal Antibody (Catalog # MAB32652) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).Applications for Human Mesothelin MAb (Clone 420425)
Application
Recommended Usage
ELISA
This antibody functions as an ELISA capture antibody when paired with Rat Anti-Human Mesothelin Monoclonal Antibody (Catalog # MAB32652).
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Mesothelin DuoSet ELISA Kit (Catalog # DY3265) for convenient development of a sandwich ELISA or the Human Mesothelin Quantikine ELISA Kit (Catalog # DMSLN0) for a complete optimized ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Mesothelin
Mesothelin is a 40 kDa glycosylphosphatidylinositol-anchored glycoprotein that is expressed on mesothelial cells in the pleura, pericardium and peritoneum and overexpressed in mesotheliomas and ovarian or pancreatic adenocarcinoma. Mesothelin is a product of the CAK-1 gene, which also encodes megakaryocyte-potentiating factor (MPF). Mature human mesothelin shares 60% amino acid identity with either mouse or rat mesothelin. Two variant forms exist; variant 1 has an eight amino acid (1 kDa) insertion and is rarely expressed, while variant 2 is a truncated form secreted in the majority of ovarian cancers but rarely found in normal individuals.
Alternate Names
CAK1, MPF, MSLN, SMR
Gene Symbol
MSLN
UniProt
Additional Mesothelin Products
Product Documents for Human Mesothelin MAb (Clone 420425)
Product Specific Notices for Human Mesothelin MAb (Clone 420425)
For research use only
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