Human Mesothelin Propeptide/MPF Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB3265

R&D Systems, part of Bio-Techne
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MAB3265
MAB3265-SP
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 346633

View all Mesothelin Antibodies »

Product Summary for Human Mesothelin Propeptide/MPF Antibody

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Mesothelin propeptide
Arg35-Leu289
Accession # AAH09272

Specificity

Detects human Mesothelin Propeptide/MPF in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human Mesothelin is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Human Mesothelin Propeptide/MPF Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human Mesothelin Propeptide/MPF
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Mesothelin

Megakaryocyte Potentiating Factor (MPF) is a 33 kDa soluble protein that is cleaved as an N-terminal propeptide from the mouse mesothelin preproprotein by furin, while the C-terminal portion is processed further to form mesothelin. Mouse MPF potentiates megakaryocyte colony forming activity of IL-3 in mouse bone marrow culture. Human MPF shows 55% amino acid identity with mouse or rat MPF. The preproprotein is produced by mesothelial cells and is also highly expressed in pancreatic, gastric and ovarian carcinomas.

Alternate Names

CAK1, MPF, MSLN, SMR

Entrez Gene IDs

10232 (Human); 56047 (Mouse); 60333 (Rat); 102130460 (Cynomolgus Monkey)

Gene Symbol

MSLN

UniProt

AAH09272

Additional Mesothelin Products

Product Documents for Human Mesothelin Propeptide/MPF Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Mesothelin Propeptide/MPF Antibody

For research use only

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