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Human Meteorin-like/METRNL Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB78672

Recombinant Monoclonal Antibody.
R&D Systems, part of Bio-Techne
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MAB78672-100
MAB78672-SP

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry, Immunohistochemistry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2441D

Product Specifications

Immunogen

Chinese Hamster Ovary cell line, CHO-derived human Meteorin-like/METRNL
Gln46-Asp311
Accession # Q641Q3

Specificity

Detects human Meteorin-like/METRNL in direct ELISAs.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human Meteorin-like/METRNL Antibody

Meteorin-like/METRNL antibody in U937 Human Cell Line by Immunocytochemistry (ICC).

Meteorin‑like/METRNL in U937 Human Cell Line.

Meteorin-like/METRNL was detected in immersion fixed U937 human histiocytic lymphoma cell line (positive staining) and U266 human myeloma cell line (negative staining) using Rabbit Anti-Human Meteorin-like/METRNL Monoclonal Antibody (Catalog # MAB78672) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Meteorin-like/METRNL antibody in Human Skeletal Muscle by Immunohistochemistry (IHC-P).

Meteorin‑like/METRNL in Human Skeletal Muscle.

Meteorin-like/METRNL was detected in immersion fixed paraffin-embedded sections of human skeletal muscle using Rabbit Anti-Human Meteorin-like/METRNL Monoclonal Antibody (Catalog # MAB78672) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to sarcoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human Meteorin-like/METRNL Antibody

Application
Recommended Usage

Immunocytochemistry

3-25 µg/mL
Sample: Immersion fixed U937 human histiocytic lymphoma cell line

Immunohistochemistry

3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human skeletal muscle

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Meteorin-like/METRNL

METRNL (Meteorin-like) is a cytoplasmic 30 kDa (predicted) member of the meteorin family of proteins. The name meteorin derives from the ability of the founding family member meteorin to transform glia into cells containing an extensive, meteor-like tail. It is expressed by neurons, and appears to function as a primer molecule that sets the stage for either cell differentiation or neurite outgrowth when it acts with Dclk1 and SerpinB1. Mature human METRNL is 266 amino acids (aa) in length (aa 46-311). It reportedly contains a signal sequence (aa 1-45), which suggests it may be secreted. Mature human and mouse METRNL share 79% aa sequence identity.

Alternate Names

Meteorin like, METRNL

Entrez Gene IDs

284207 (Human); 210029 (Mouse); 316842 (Rat)

Gene Symbol

METRNL

UniProt

Additional Meteorin-like/METRNL Products

Product Documents for Human Meteorin-like/METRNL Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Meteorin-like/METRNL Antibody

For research use only

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