Human Meteorin-like/METRNL Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB78672
Recombinant Monoclonal Antibody.
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry, Immunohistochemistry
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2441D
Product Specifications
Immunogen
Chinese Hamster Ovary cell line, CHO-derived human Meteorin-like/METRNL
Gln46-Asp311
Accession # Q641Q3
Gln46-Asp311
Accession # Q641Q3
Specificity
Detects human Meteorin-like/METRNL in direct ELISAs.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human Meteorin-like/METRNL Antibody
Meteorin‑like/METRNL in U937 Human Cell Line.
Meteorin-like/METRNL was detected in immersion fixed U937 human histiocytic lymphoma cell line (positive staining) and U266 human myeloma cell line (negative staining) using Rabbit Anti-Human Meteorin-like/METRNL Monoclonal Antibody (Catalog # MAB78672) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Meteorin‑like/METRNL in Human Skeletal Muscle.
Meteorin-like/METRNL was detected in immersion fixed paraffin-embedded sections of human skeletal muscle using Rabbit Anti-Human Meteorin-like/METRNL Monoclonal Antibody (Catalog # MAB78672) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to sarcoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human Meteorin-like/METRNL Antibody
Application
Recommended Usage
Immunocytochemistry
3-25 µg/mL
Sample: Immersion fixed U937 human histiocytic lymphoma cell line
Sample: Immersion fixed U937 human histiocytic lymphoma cell line
Immunohistochemistry
3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human skeletal muscle
Sample: Immersion fixed paraffin-embedded sections of human skeletal muscle
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Meteorin-like/METRNL
Alternate Names
Meteorin like, METRNL
Gene Symbol
METRNL
UniProt
Additional Meteorin-like/METRNL Products
Product Documents for Human Meteorin-like/METRNL Antibody
Product Specific Notices for Human Meteorin-like/METRNL Antibody
For research use only
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