Human MFAP3 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB10798

R&D Systems, part of Bio-Techne
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MAB10798-100
MAB10798-SP
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1059216

View all MFAP3 Antibodies »

Product Specifications

Immunogen

Human embryonic kidney cell, HEK293-derived human MFAP3
Leu22-Met147
Accession # P55082

Specificity

Detects human MFAP3 in direct ELISA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human MFAP3 Antibody

Detection of MFAP3 in Human Tonsil.

MFAP3 was detected in immersion fixed paraffin-embedded sections of Human Tonsil using Mouse Anti-Human MFAP3 Monoclonal Antibody (Catalog # MAB10798) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human MFAP3 Antibody

Application
Recommended Usage

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of Human Tonsil

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MFAP3

Microfibril-associated glycoprotein 3 (MFAP3), is a member of the MFAP family which play a role in microfibril assembly, elastinogenesis, and tissue homeostasis. The family members were identified as potential components of fibrillin-containing microfibrils, but they do not share structural similarities (1). MFAP3 is found in ocular zonules. Human MFAP3 is a serine-rich acidic protein located in zonular fibers and consists of an extracellular domain (ECD) containing one Ig-like C2-type domain, a single transmembrane domain, and a cytoplasmic domain. Within the ECD, mature human MFAP3 shares 74% amino acid sequence identity with mouse and rat MFAP3. Among MFAP3-related pathways are degradation of the extracellular matrix and elastic fiber formation. MFAP is a candidate gene for heritable diseases affecting microfibrils (2).

References

  1. Gibson, M.A. Landes Bioscience https://www.ncbi.nlm.nih.gov/books/NBK6448/.
  2. Abrams W.R. (1995) Genomics 26:47.

Long Name

Microfibrillar-Associated Protein 3

Alternate Names

DKFZp586F0219, microfibril-associated glycoprotein 3, microfibrillar-associated protein 3

Entrez Gene IDs

4238 (Human); 216760 (Mouse); 287299 (Rat)

Gene Symbol

MFAP3

UniProt

P55082

Additional MFAP3 Products

Product Documents for Human MFAP3 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human MFAP3 Antibody

For research use only

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