HEK293 human embryonic kidney cell line transfected with (A) human mGLUR2 or (B) irrelevant protein and eGFP was stained with Mouse Anti-Human mGLUR2 Monoclonal Antibody (Catalog # MAB46762) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins.

mGluR2 in Rat Brain.

mGluR2 was detected in immersion fixed paraffin-embedded sections of rat brain (cerebellum) using Mouse Anti-Human mGluR2 Monoclonal Antibody (Catalog # MAB46762) at 8 µg/mL for 1 hour at room temperature followed by incubation with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (NL007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface of neurons in granular cell layer. Staining was performed using our protocol for Fluorescent IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human mGluR2 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 Human Cell Line Transfected with Human mGLUR2 and eGFP

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffinembedded sections of rat brain (cerebellum)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: mGluR2

Metabotropic glutamate receptor 2 (mGluR2) is an 872 aa, predicted 96 kDa multipass G protein coupled inhibitory receptor belonging to group II of the metabotropic glutamate receptor family. It is localized largely on the presynaptic side of glutamatergic and other neurotransmitter synapses in areas of the forebrain. mGluR2 activity is potentially involved in some anxiety disorders. The long N‑terminal extracellular region of human mGluR2 (aa 1‑498) shares 97% aa identity with either mouse or rat mGluR2.

Long Name

Metabotropic Glutamate Receptor 2

Alternate Names

GPRC1B, GRM2, mGluR7

Entrez Gene IDs

2912 (Human); 108068 (Mouse); 24415 (Rat)

Gene Symbol

GRM2

UniProt

Q14416

Additional mGluR2 Products

Product Documents for Human mGluR2 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human mGluR2 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human mGluR2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB46762

Key Product Details

Species Reactivity

Validated:

Applications

Label

Antibody Source

View all mGluR2 Antibodies »

Product Summary for Human mGluR2 Antibody

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human mGluR2 Antibody

Detection of mGLUR2 in HEK293 Human Cell Line Transfected with Human mGLUR2 and eGFP by Flow Cytometry.