Human mGluR2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB46761
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 455311
Product Summary for Human mGluR2 Antibody
Immunogen
Mouse myeloma cell line, NS0-derived human mGluR2
Glu19-Ser498
Accession # Q14416
Glu19-Ser498
Accession # Q14416
Specificity
Detects human mGluR2 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human mGluR2 Antibody
mGluR2 in Human Brain.
mGluR2 was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using Mouse Anti-Human mGluR2 Monoclonal Antibody (Catalog # MAB46761) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes in Purkinje neurons and in granular cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human mGluR2 Antibody
Application
Recommended Usage
Immunohistochemistry
5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cerebellum)
Sample: Immersion fixed paraffin-embedded sections of human brain (cerebellum)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: mGluR2
Metabotropic glutamate receptor 2 (mGluR2, also known as GPRC1B and GRM2) is an 872 aa, predicted 96 kDa multipass G protein coupled inhibitory receptor (GPCR) belonging to group II of the metabotropic glutamate receptor family. The receptor functions as an autoreceptor for glutamate, that upon activation, inhibits the emptying of vesicular contents at the presynaptic terminal of glutamatergic neurons. It is localized largely on the presynaptic side of glutamatergic and other neurotransmitter synapses in areas of the forebrain. mGluR2 activity is potentially involved in some anxiety disorders. The long N‑terminal extracellular region of human mGluR2 (aa 1‑498) shares 97% aa identity with either mouse or rat mGluR2.
Long Name
Metabotropic Glutamate Receptor 2
Alternate Names
GPRC1B, GRM2, mGluR7
Gene Symbol
GRM2
UniProt
Additional mGluR2 Products
Product Documents for Human mGluR2 Antibody
Product Specific Notices for Human mGluR2 Antibody
For research use only
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