Human mGluR3 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4668
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Specifications
Immunogen
Asp25-Ser507
Accession # Q14832
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human mGluR3 Antibody
Detection of MGLUR3 in HEK293 Human Cell Line Transfected with Human MGLUR3 and eGFP by Flow Cytometry.
HEK293 human embryonic kidney cell line transfected with human MGLUR3 and eGFP was stained with (A) Mouse Anti-Human MGLUR3 Monoclonal Antibody (Catalog # MAB4668) or (B) Mouse IgG2B isotype control antibody (Catalog # MAB0041) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.mGluR3 in Human Hypothalamus Tissue.
mGluR3 was detected in immersion fixed paraffin-embedded sections of human hypothalamus tissue using Mouse Anti-Human mGluR3 Monoclonal Antibody (Catalog # MAB4668) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membrane in neurons. Staining was performed using our IHC Staining with VisUCyte HRP Polymer Detection Reagents protocol.Applications for Human mGluR3 Antibody
CyTOF-ready
Flow Cytometry
Sample: HEK293 Human Cell Line Transfected with Human MGLUR3 and eGFP
Immunohistochemistry
Sample: Immersion fixed paraffin-embedded sections of human hypothalamus tissue
Formulation, Preparation, and Storage
Purification
Reconstitution
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: mGluR3
Metabotropic glutamate receptor 3 (mGluR3) is a 90-100 kDa, 7-transmembrane glycoprotein that belongs to group II of the C-family of G-protein coupled receptors. It is a presynaptic receptor expressed on both neurons and glia, whose activation reduces adenylate cyclase activity. Mature human mGluR3 is 857 amino acids in length and contains a 554 amino acid (aa) N-terminal extracellular domain (ECD) (aa 23-576). The ECD binds glutamate and forms homodimers. There is one alternative splice form that is soluble, 515 aa in length and shows a 96 aa substitution for aa 442-879. Over aa 25-507, human mGluR3 shares 97% aa sequence identity with mouse and rat mGluR3 and 67% aa sequence identity with hGluR2.
Long Name
Alternate Names
Gene Symbol
UniProt
Additional mGluR3 Products
Product Documents for Human mGluR3 Antibody
Product Specific Notices for Human mGluR3 Antibody
For research use only