Human MICL/CLEC12A Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2946
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human MICL/CLEC12A isoform a
Thr67-Ala265
Accession # NP_612210
Thr67-Ala265
Accession # NP_612210
Specificity
Detects human MICL/CLEC12A in direct ELISAs and Western blots. In Western blots, approximately 15% cross-reactivity with recombinant mouse MICL is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human MICL/CLEC12A Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed activated human peripheral blood mononuclear cells
Sample: Immersion fixed activated human peripheral blood mononuclear cells
Western Blot
0.1 µg/mL
Sample: Recombinant Human MICL/CLEC12A
Sample: Recombinant Human MICL/CLEC12A
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MICL/CLEC12A
MICL, also known as C-type lectin-like molecule-1 (CLL-1), Killer cell lectin-like receptor KLRL1 and C type lectin domain family 12, member A (CLEC12A), is a type II transmembrane highly glycosylated protein that contains a functional tyrosine-based inhibitory motif in its cytoplasmic tail. The gene for MICL has been mapped to the natural killer gene complex (NKC) on human chromosome 12p13.2. MICL contains a non-classic C-type domain that binds non-sugar ligands. It is primarily expressed on NK cells, T cells, dendritic cells, monocytes and macrophages. By alternative splicing, multiple splice isoforms exist. MICL is a negative regulator of granulocytes and monocyte function and inhibits NK cell cytotoxicity. The amino acid sequence of human MICL extracellular domain is 53%, 65% and 55% identical to that of mouse, canine and bovine MICL, respectively.
Long Name
Myeloid Inhibitory C-type Lectin-like Receptor
Alternate Names
CD371, CLEC12A, CLL-1, DCAL-2
Gene Symbol
CLEC12A
UniProt
Additional MICL/CLEC12A Products
Product Documents for Human MICL/CLEC12A Antibody
Product Specific Notices for Human MICL/CLEC12A Antibody
For research use only
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