Human MIG2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6984
Key Product Details
Species Reactivity
Validated:
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 696906
Product Summary for Human MIG2 Antibody
Immunogen
E. coli-derived recombinant human MIG2
Gln321-Leu444
Accession # Q96AC1
Gln321-Leu444
Accession # Q96AC1
Specificity
Detects human MIG2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) MIG, rhFERMT1 (aa 514‑677), rhMIG2/FERMT2 (aa 514‑680), or rhFERMT3 (498‑667) is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human MIG2 Antibody
Detection of Human MIG2 by Western Blot.
Western blot shows lysates of HT1080 human fibrosarcoma cell line, A549 human lung carcinoma cell line, and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human MIG2 Monoclonal Antibody (Catalog # MAB6984) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for MIG2 at approximately 78 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human MIG2 Antibody
Application
Recommended Usage
Western Blot
0.5 µg/mL
Sample: HT1080 human fibrosarcoma cell line, A549 human lung carcinoma cell line, and HeLa human cervical epithelial carcinoma cell line
Sample: HT1080 human fibrosarcoma cell line, A549 human lung carcinoma cell line, and HeLa human cervical epithelial carcinoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MIG2
Long Name
Mitogen Inducible Gene 2
Alternate Names
FERMT2, KIND2, Kindlin-2, PLEKHC1
Gene Symbol
FERMT2
UniProt
Additional MIG2 Products
Product Documents for Human MIG2 Antibody
Product Specific Notices for Human MIG2 Antibody
For research use only
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