Human Mind Bomb 2/MIB2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB72891

R&D Systems, part of Bio-Techne
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MAB72891
MAB72891-SP
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 739505

View all Mind Bomb 2/MIB2 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Mind Bomb 2/MIB2
Ala418-Lys520
Accession # Q96AX9

Specificity

Detects human Mind Bomb 2/MIB2 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Mind Bomb 2/MIB2 Antibody

Detection of Human Mind Bomb 2/MIB2 antibody by Western Blot.

Detection of Human Mind Bomb 2/MIB2 by Western Blot.

Western blot shows lysates of U266 human myeloma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Mind Bomb 2/MIB2 Monoclonal Antibody (Catalog # MAB72891) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Mind Bomb 2/MIB2 at approximately 103-110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Mind Bomb 2/MIB2 Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: U266 human myeloma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Mind Bomb 2/MIB2

MIB-2 (Mind Bomb-2), also called skeletrophin, is a widely expressed, 1013 amino acid (aa) cytoplasmic phosphoprotein that is an E3 ubiquitin ligase for Notch ligands such as Jagged-2. It contains two MIB/HERC2 domains surrounding a ZZ-type zinc finger (aa 59-285), an ankyrin repeat domain (aa 522-856), and two RING-type zinc fingers that have E3 ubiquitin ligase activity (aa 890-1002). If short gaps in the protein sequence are ignored, human MIB-2 (aa 418-520) shares 95% and 92% aa identity with mouse and rat MIB-2, respectively. Eight alternately spliced isoforms of 672-1009 aa lack various sequences, but all contain aa 418-520. MIB-2 has been detected as a 70 kDa form in skeletal muscle, binds actin, and is downregulated in malignant melanoma.

Long Name

Mindbomb Homolog 2

Alternate Names

MIB2, Mind Bomb-2, Novelzin, SKD, Skeletrophin, ZZANK1, ZZZ5

Entrez Gene IDs

142678 (Human); 76580 (Mouse)

Gene Symbol

MIB2

UniProt

Q96AX9 (Human)

Additional Mind Bomb 2/MIB2 Products

Product Documents for Human Mind Bomb 2/MIB2 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Mind Bomb 2/MIB2 Antibody

For research use only

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