Human MKK7 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3579
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 324302
Product Specifications
Immunogen
E. coli-derived recombinant human MKK7
Met1-Arg419
Accession # O14733
Met1-Arg419
Accession # O14733
Specificity
Detects rhMKK7 in direct ELISAs. This antibody does not cross-react with rhMKK4.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human MKK7 Antibody
MKK7 in Human Tonsil.
MKK7 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human MKK7 Monoclonal Antibody (Catalog # MAB3579) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human MKK7 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human skeletal muscle and tonsil
Sample: Immersion fixed paraffin-embedded sections of human skeletal muscle and tonsil
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MKK7
Mitogen-activated protein kinase kinase 7 (MKK7 or MAP2K7), also known as Jun kinase kinase 2 (JNKK2), is activated by proinflammatory cytokines and environmental stresses. Activation occurs through phosphorylation at Ser 271 and Thr 275 by several upstream MAPK kinase kinases (MAP3Ks). MKK7 is a dual specificity protein kinase, phosphorylating and activating the JNK family of MAP kinases at Thr and Tyr positions within the phosphoacceptor sequence Thr-Pro-Tyr.
Long Name
Mitogen-activated Protein Kinase Kinase 7
Alternate Names
JNKK2, MAP2K7, MKE7, PRKMK7
Gene Symbol
MAP2K7
UniProt
Additional MKK7 Products
Product Documents for Human MKK7 Antibody
Product Specific Notices for Human MKK7 Antibody
For research use only
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