Human MKP-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7168
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 408612
Product Summary for Human MKP-1 Antibody
Immunogen
E. coli-derived recombinant human MKP-1
Met1-Cys367
Accession # P28562
Met1-Cys367
Accession # P28562
Specificity
Detects human MKP-1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) MKP-2, rhMKP-3, or rhMKP-5 is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Human MKP-1 Antibody
MKP‑1 in Human Prostate.
MKP-1 was detected in immersion fixed paraffin-embedded sections of human prostate using Rat Anti-Human MKP-1 Monoclonal Antibody (Catalog # MAB7168) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei and cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human MKP-1 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human prostate
Sample: Immersion fixed paraffin-embedded sections of human prostate
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MKP-1
Long Name
MAP Kinase Phosphatase 1
Alternate Names
CL100, DUSP1, HVH1, MKP1, PTPN10
Gene Symbol
DUSP1
UniProt
Additional MKP-1 Products
Product Documents for Human MKP-1 Antibody
Product Specific Notices for Human MKP-1 Antibody
For research use only
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