Human MLK3 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4559
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human MLK3
Ala85-Gly207
Accession # Q16584
Ala85-Gly207
Accession # Q16584
Specificity
Detects endogenous human MLK3 in Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human MLK3 Antibody
Detection of Human MLK3 by Western Blot.
Western blot shows lysates of Ramos human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human MLK3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4559) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for MLK3 at approximately 93 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.Applications for Human MLK3 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Ramos human Burkitt's lymphoma cell line
Sample: Ramos human Burkitt's lymphoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MLK3
Mixed-lineage kinases (MLKs) function as mitogen-activated protein kinase kinase kinases (MAP3Ks), activating the MAPK signaling cascades. MLK3, also known as MAP3K11 and PTK1, encodes a protein of 847 amino acids (aa) that shares an unusual leucine zipper‑basic motif found in other MLKs. Small interfering RNA experiments have indicated that MLK3 is required for both mitogen and cytokine activation of JNK1, p38 alpha, and ERK1.
Long Name
Mixed Lineage Kinase 3
Alternate Names
MAP3K11, PTK1, RHOE, SPRK
Gene Symbol
MAP3K11
UniProt
Additional MLK3 Products
Product Documents for Human MLK3 Antibody
Product Specific Notices for Human MLK3 Antibody
For research use only
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