Human MLX Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4186
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Mouse
Applications
Validated:
Immunocytochemistry, Western Blot
Cited:
Chromatin Immunoprecipitation (ChIP)
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human MLX
Glu197-Tyr298
Accession # EAW60842
Glu197-Tyr298
Accession # EAW60842
Specificity
Detects human MLX in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human MLX Antibody
Detection of Human MLX by Western Blot.
Western blot shows nuclear extracts of PC-3 human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human MLX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4186) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for MLX at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
MLX in U2OS Human Cell Line.
MLX was detected in immersion fixed U2OS human osteosarcoma cell line using Goat Anti-Human MLX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4186) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cell nuclei. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Human MLX Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed U2OS human osteosarcoma cell line
Sample: Immersion fixed U2OS human osteosarcoma cell line
Western Blot
1 µg/mL
Sample: PC-3 human prostate cancer cell line
Sample: PC-3 human prostate cancer cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MLX
MLX (Max-like protein X; also known as transcription factor-like protein 4) is a 30 kDa member of the Max-like bHLHZip family of proteins. It is widely expressed and is found in both cytoplasm and nucleus. MLX is a transcriptional repressor when dimerized with MAD family members and a transcriptional activator when dimerized with MONDO family members. Human MLX is 298 amino acids (aa) in length. It contains one DNA-binding region (aa 76‑87), an HLH domain (aa 127‑192) and a Leu-zipper domain (aa 140‑160). There are three alternate splice short forms. One shows the deletion of aa 15‑68, a second shows deletion of aa 15‑68 and 81‑110, and a third shows a 3 aa substitution for aa 111‑298. Over aa 197‑298, human MLX is 99% identical to mouse and canine MLX.
Long Name
MAX-like Protein X
Alternate Names
BigMax alpha, MAD7, MXD7, TCFL4
Gene Symbol
MLX
UniProt
Additional MLX Products
Product Documents for Human MLX Antibody
Product Specific Notices for Human MLX Antibody
For research use only
Loading...
Loading...
Loading...
Loading...