Human MMP-13 Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF511
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot
Cited:
ELISA Detection
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human MMP-13 (R&D Systems, Catalog # 511-MM)
Leu20-Cys471
Accession # P45452
Leu20-Cys471
Accession # P45452
Specificity
Detects human MMP-13 in Western blots. In this format, approximately 5% cross-reactivity with recombinant human (rh) MMP‑8 is observed and less than 2% cross-reactivity with rhMMP-1, -2, -3, -7, -9, and -12 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human MMP-13 Biotinylated Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Human MMP‑13 (Catalog # 511-MM)
Sample: Recombinant Human MMP‑13 (Catalog # 511-MM)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MMP-13
Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-13 (Collagenase-3) has been demonstrated to degrade a range of extracellular matrix proteins, including collagen types I, II, III, IV, IX, X and XIV, gelatin, aggrecan, perlecan and fibronectin. MMP-13 is distinguished from the other human collagenases by its effecient degradation of type II collagen. MMP-13 is expressed by fibroblasts, chrondrocytes and squamous epithelial cells. Structurally, MMP-13 may be divided into several distinct domains; a pro-domain which is cleaved upon activation; a catalytic domain containing the zinc binding site; a short hinge region and a carboxyl terminal (hemopexin-like) domain.
References
- Jeffery, J.J. (1998) in Collagenase 3. A.J. Barrett, et al. (eds): Handbook of Proteolytic Enzymes, San Diego: Academic Press, p. 1167.
Long Name
Matrix Metalloproteinase 13
Alternate Names
MMP13
Entrez Gene IDs
4322 (Human)
Gene Symbol
MMP13
UniProt
Additional MMP-13 Products
Product Documents for Human MMP-13 Biotinylated Antibody
Product Specific Notices for Human MMP-13 Biotinylated Antibody
For research use only
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