Human MMP-2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB9023
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 101719
Product Summary for Human MMP-2 Antibody
Immunogen
Mouse myeloma cell line NS0-derived recombinant human MMP‑2
Ala30-Cys660
Accession # P08253
Ala30-Cys660
Accession # P08253
Specificity
Detects human MMP-2 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human MMP-2 Antibody
Human MMP‑2 ELISA Standard Curve.
Recombinant Human MMP-2 protein was serially diluted 2-fold and captured by Mouse Anti-Human MMP-2 Monoclonal Antibody (Catalog # MAB9024) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human MMP-2 Monoclonal Antibody (Catalog # MAB9023) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).Applications for Human MMP-2 Antibody
Application
Recommended Usage
ELISA
This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human MMP‑2 Monoclonal Antibody (Catalog # MAB9024).
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human MMP-2 DuoSet ELISA Kit (Catalog # DY902) for convenient development of a sandwich ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from ascites
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MMP-2
Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-2 (gelatinase A), a type IV collagenase, can degrade a broad range of substrates including type IV, V, VII and X collagens as well as elastin and fibronectin. It is believed to act synergistically with interstitial collagenase (MMP-1) in the degradation of fibrillar collagens as it degrades their denatured gelatin forms. MMP-2 has been shown to be associated with many connective tissue cells as well as neutrophils, macrophages and monocytes. Structurally, MMP-2 may be divided into several distinct domains: a pro-domain which is cleaved upon activation; a catalytic domain containing the zinc binding site; a fibronectin-like domain thought to play a role in substrate targeting; and a carboxyl terminal (hemopexin-like) domain containing 2 N-linked glycosylation sites.
Long Name
Matrix Metalloproteinase 2
Alternate Names
Gelatinase A, MMP2
Gene Symbol
MMP2
UniProt
Additional MMP-2 Products
Product Documents for Human MMP-2 Antibody
Product Specific Notices for Human MMP-2 Antibody
For research use only
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