Detects the pro and active forms of human MMP-2 in Western blots. In dot blots, no cross-reactivity with recombinant human MMP-1, -3, -7,-8, -9, -10, -12, or -13 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2A

Applications for Human MMP-2 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

2.5 µg/10⁶ cells
Sample: MG-63 human osteosarcoma cell line fixed with paraformaldehyde and permeabilized with saponin

Western Blot

1 µg/mL
Sample: Recombinant Human MMP-2 Western Blot Standard (Catalog # WBC025)
under non-reducing conditions only

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MMP-2

Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP‑2 (gelatinase A), a type IV collagenase, can degrade a broad range of substrates including type IV, V, VII and X collagens as well as elastin and fibronectin. It is believed to act synergistically with interstitial collagenase (MMP‑1) in the degradation of fibrillar collagens as it degrades their denatured gelatin forms. MMP‑2 has been shown to be associated with many connective tissue cells as well as neutrophils, macrophages and monocytes. Structurally, MMP‑2 may be divided into several distinct domains: a pro-domain which is cleaved upon activation; a catalytic domain containing the zinc binding site; a fibronectin-like domain thought to play a role in substrate targeting; and a carboxyl terminal (hemopexin-like) domain containing 2 N-linked glycosylation sites.

Long Name

Matrix Metalloproteinase 2

Alternate Names

Gelatinase A, MMP2

Entrez Gene IDs

4313 (Human); 17390 (Mouse)

Gene Symbol

MMP2

Additional MMP-2 Products

Product Documents for Human MMP-2 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human MMP-2 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human MMP-2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB903

Key Product Details

Species Reactivity

Validated:

Cited:

Applications