Human MMP-2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF902
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Canine, Fish - Danio rerio (Zebrafish), Rabbit
Applications
Validated:
Immunohistochemistry, Immunoprecipitation, Simple Western, Western Blot
Cited:
ELISA Microarray Development, Immunocytochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human MMP-2
Ile34-Cys660
Accession # P08253
Ile34-Cys660
Accession # P08253
Specificity
Detects human MMP-2 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human MMP-2 Antibody
Detection of Human MMP-2 by Western Blot.
Western blot shows lysate of U-118-MG human glioblastoma/astrocytoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human MMP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF902) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for MMP-2 at approximately 72 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
MMP‑2 in Human Ovarian Cancer Tissue.
MMP-2 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using Goat Anti-Human MMP-2 Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF902) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counter-stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
MMP‑2 in Human Ovary.
MMP-2 was detected in immersion fixed paraffin-embedded sections of human ovarian array using Goat Anti-Human MMP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF902) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human MMP-2 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human ovarian cancer tissue and normal human ovarian array
Sample: Immersion fixed paraffin-embedded sections of human ovarian cancer tissue and normal human ovarian array
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human MMP-2 (Catalog # 902-MP), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human MMP-2 (Catalog # 902-MP), see our available Western blot detection antibodies
Simple Western
10 µg/mL
Sample: U‑118‑MG human glioblastoma/astrocytoma cell line
Sample: U‑118‑MG human glioblastoma/astrocytoma cell line
Western Blot
1 µg/mL
Sample: U‑118‑MG human glioblastoma/astrocytoma cell line
Sample: U‑118‑MG human glioblastoma/astrocytoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 4 reviews rated 4.3 using AF902 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MMP-2
Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-2 (gelatinase A), a type IV collagenase, can degrade a broad range of substrates including type IV, V, VII and X collagens as well as elastin and fibronectin. It is believed to act synergistically with interstitial collagenase (MMP-1) in the degradation of fibrillar collagens as it degrades their denatured gelatin forms. MMP-2 has been shown to be associated with many connective tissue cells as well as neutrophils, macrophages and monocytes. Structurally, MMP-2 may be divided into several distinct domains: a pro-domain which is cleaved upon activation; a catalytic domain containing the zinc binding site; a fibronectin-like domain thought to play a role in substrate targeting; and a carboxyl terminal (hemopexin-like) domain containing 2 N-linked glycosylation sites.
Long Name
Matrix Metalloproteinase 2
Alternate Names
Gelatinase A, MMP2
Gene Symbol
MMP2
UniProt
Additional MMP-2 Products
Product Documents for Human MMP-2 Antibody
Product Specific Notices for Human MMP-2 Antibody
For research use only
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